Evidence map›Paper›PMID 35482182›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Yeast Surface Display: New Opportunities for a Time-Tested Protein Engineering System.

Maryam Raeeszadeh-Sarmazdeh, Eric T Boder

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Review
  7. Engineering Selective TIMPs Using a Counter-Selective Screening Strategy.Methods in molecular biology (Clifton, N.J.) · 2024
    Article
  8. Article
  9. Frontiers in bioengineering and biotechnology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Maryam Raeeszadeh-SarmazdehDepartment of Chemical and Materials Engineering, University of Nevada, Reno, NV, USA. maryamr@unr.edu.
Eric T BoderDepartment of Chemical and Biomolecular Engineering, University of Tennessee, Knoxville, TN, USA. boder@utk.edu.

Funding

Virtual Reality and Augmented Reality CoreP20GM103650 · NIGMS · UNIVERSITY OF NEVADA RENO · PI WEBSTER, MICHAEL A · 2012 to 2022
$20.9M
Understanding and Controlling Adhesive Protein Affinity SwitchingR15GM122326 · NIGMS · UNIVERSITY OF TENNESSEE KNOXVILLE · PI BODER, ERIC T · 2016 to 2016
$427k
Bio-Layer Interferometry (BLI) Octet R8S10OD032385 · OD · UNIVERSITY OF NEVADA RENO · PI RAEESZADEH SARMAZDEH, MARYAM · 2023 to 2023
$313k
Directed evolution of tissue inhibitor of metalloproteinase 3 (TIMP-3) to develop novel Alzheimer’s disease (AD) therapeuticsR03AG070511 · NIA · UNIVERSITY OF NEVADA RENO · PI RAEESZADEH SARMAZDEH, MARYAM · 2022 to 2022
$300k
NIA NIH HHS R03 AG070511NIGMS NIH HHS P20 GM103650NIGMS NIH HHS R15 GM122326NIH HHS S10 OD032385
6 · The paper itself

Abstract

Yeast surface display has proven to be a powerful tool for the discovery of antibodies and other novel binding proteins and for engineering the affinity and selectivity of existing proteins for their targets. In the decades since the first demonstrations of the approach, the range of yeast display applications has greatly expanded to include many different protein targets and has grown to encompass methods for rapid protein characterization. Here, we briefly summarize the development of yeast display methodologies and highlight several selected examples of recent applications to timely and challenging protein engineering and characterization problems.

Indexed as

Peptide LibrarySaccharomyces cerevisiaeAntibodiesProtein EngineeringAntibodiesPeptide LibraryAntibody engineeringDeep mutational scanningDirected evolutionIn vitro antibody selectionsLibrary screeningProtein engineeringProtein therapeuticsYeast display

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.