The restricted adhesion of bone marrow mesenchymal stem cells by stepped structures on surfaces of hydroxyapatite.

Currently, many researches have developed several strategies to design the surface structures of hydroxyapatite (HA), and have proved that the surface structures are pivotal in guiding the adhesion of bone marrow mesenchymal stem cells (BMSCs) as well as subsequent cellular behaviours. Most of these strategies, such as altering roughness and constructing surface patterning of HA, involve the construction of geometric topographies at the micro/nanoscale. However, besides geometric topographies, crystal defects are also important characteristics of surface structures and would alter many local physicochemical properties, which is critical for contact between cells and bioceramic surfaces. For the practical applications of crystal defects, a major hindrance is that crystal defects are usually unstable and easily eliminated during crystallization, which limits the large-scale fabrication of materials with crystal defects. In this work, given that stepped structures contain massive stable crystal defects on their step edges and kinks, we proposed a feasible and efficient method to fabricate HA dishes with stepped structures on their surfaces. First, plate-like HA mesocrystals were prepared from CaHPO