Evidence map›Paper›PMID 35478597›Full record

ArticleMethodsX2022

Craniotomy for acute monitoring of pial vessels in the rodent brain.

Refat Aboghazleh, Baraah Alkahmous, Evyatar Swissa, Saara Mansoor, Alon Friedman, Ofer Prager

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MethodsX, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 4 countries.

Refat AboghazlehDepartment of Basic Medical Sciences, Faculty of Medicine, Al-Balqa Applied University, Al-Salt, Jordan.
Baraah AlkahmousDepartment of Internal Medicine, Prince Mohammed Bin Abdulaziz Hospital, Riyadh, Saudi Arabia.
Evyatar SwissaDepartments of Physiology and Cell Biology, Cognitive and Brain Sciences, Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Saara MansoorDepartment of Medical Neuroscience, Faculty of Medicine and Brain Repair Center, Dalhousie University, Halifax, Canada.
Alon FriedmanDepartments of Physiology and Cell Biology, Cognitive and Brain Sciences, Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Ofer PragerDepartments of Physiology and Cell Biology, Cognitive and Brain Sciences, Zlotowski Center for Neuroscience, Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Ben-Gurion University of the Negev · ILDalhousie University · CAPrince Mohammed bin Abdulaziz Hospital · SA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A growing awareness for vascular contribution to pathogenesis of brain diseases increases the need for techniques that allow high-resolution imaging and quantification of changes in function and structure of cerebral microvessels. Cerebral vessels are very sensitive structures, making them vulnerable for injury. In addition, they are uniquely characterized with the blood-brain barrier, and an extra caution is required during procedures that involve engagement of cerebral vessels (i.e., craniotomy). Using state of the art facilities, including 3D intravital microscope, we describe here in details:•The steps and equipment required for drilling a craniotomy and removing of the dura, while keeping brain parenchyma and vessels intact. This enables long duration of live and direct monitoring of pial vessels and imaging of BBB permeability.•We present the craniotomy procedure that relevant and compatible with imaging pial vessels and monitoring the blood-brain barrier in small rodents.

Indexed as

ACSF, artificial cerebrospinal fluidBBB, blood-brain barrierBlood-brain barrierCerebrovascular imagingCNS, central nervous systemCranial windowCraniotomyECs, endothelial cellsElectrocorticographySpO2, blood oxygen saturation

Identifiers

PMID35478597
PMCPMC9036111
OpenAlexW4224229786

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.