ArticleBone research2022
A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration.
Article in Bone research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
46 citing papers in PubMed, 64 citations in OpenAlex.
- Long-term expandable auricular chondrocytes reveal a conserved nine-gene decline module underlying senescence-independent dedifferentiation.Regenerative therapy · 2026Article
- The Effect of Physical, Biochemical, and Electrical Culture Conditions on Articular Chondrocytes Used for Cartilage Tissue Engineering: A Narrative Review.International journal of molecular sciences · 2026Review
- Integrated transcriptomic and functional analysis reveals tissue-specific molecular pathology in adolescent idiopathic scoliosis.HGG advances · 2026Article
- DHA-Derived Lipid Mediators Attenuate Osteoarthritis by Resolving Inflammation and Protecting Cartilage in Association with the SIRT1 Signaling Pathway.Marine drugs · 2026Article
- O-SNAP uncovers nanoscale chromatin remodeling in dedifferentiation and stress responses.Nature communications · 2026Article
- Microcavitary Alginate Hydrogel Reverses Chondrocyte Dedifferentiation via Temporal Modulation of the p53 Pathway.Annals of biomedical engineering · 2026Article
- Role of WIF-1 in regulating chondrocyte dedifferentiation and matrix production through WNT pathway.Regenerative therapy · 2026Article
- Semaphorin 7A Regulates the Balance Between Cartilaginous and Fibrous Tissues in the Repair Process of Articular Cartilage Damage.Cartilage · 2026Article
- Bioenergetic-active hydrogel drives extracellular matrix synthesis for cartilage repair.Bioactive materials · 2025Article
- Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis.Bone research · 2025Article
- 2D Chitosan-Based Films: A Proteomic Mass Spectrometry Study of Chondrocyte Phenotype as a Function of Cell-Biomaterial Interactions.International journal of molecular sciences · 2025Article
- Antioxidant functionalized double-net/TA dynamic hydrogel promotes cartilage regeneration through stabilization of chondrocyte phenotype.Materials today. Bio · 2025Article
- Dynamic Compression Improves Chondrogenesis in the Tissue Engineered Model of Cartilage.Biotechnology and bioengineering · 2025Article
- Accelerating cartilage regeneration with DNA-SF hydrogel sustained release system-based cartilage organoids.Military Medical Research · 2025Article
- Blocking calcium-MYC regulatory axis inhibits early dedifferentiation of chondrocytes and contributes to cartilage regeneration.Stem cell research & therapy · 2025Article
- Current cutting-edge omics techniques on musculoskeletal tissues and diseases.Bone research · 2025Review
- Tracking chondrocyte-to-fibroblast transformation via changes in cell electrophysiology.Scientific reports · 2025Article
- Fibrocartilage hyalinization: A potential therapeutic strategy for articular fibrocartilage.Journal of orthopaedic translation · 2025Review
- Exploring the role of directly coupled alternating electric fields on chondrocyte morphology and redifferentiation capacity with a focus on sex differences.Journal of experimental orthopaedics · 2025Article
- Recapitulation of endochondral ossification by hPSC-derived SOX9Nature communications · 2025Article
Corrections and comments
- Erratum issued
Authors and funding
14 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Articular cartilage damage is a universal health problem. Despite recent progress, chondrocyte dedifferentiation has severely compromised the clinical outcomes of cell-based cartilage regeneration. Loss-of-function changes are frequently observed in chondrocyte expansion and other pathological conditions, but the characteristics and intermediate molecular mechanisms remain unclear. In this study, we demonstrate a time-lapse atlas of chondrocyte dedifferentiation to provide molecular details and informative biomarkers associated with clinical chondrocyte evaluation. We performed various assays, such as single-cell RNA sequencing (scRNA-seq), live-cell metabolic assays, and assays for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), to develop a biphasic dedifferentiation model consisting of early and late dedifferentiation stages. Early-stage chondrocytes exhibited a glycolytic phenotype with increased expression of genes involved in metabolism and antioxidation, whereas late-stage chondrocytes exhibited ultrastructural changes involving mitochondrial damage and stress-associated chromatin remodeling. Using the chemical inhibitor BTB06584, we revealed that early and late dedifferentiated chondrocytes possessed distinct recovery potentials from functional phenotype loss. Notably, this two-stage transition was also validated in human chondrocytes. An image-based approach was established for clinical use to efficiently predict chondrocyte plasticity using stage-specific biomarkers. Overall, this study lays a foundation to improve the quality of chondrocytes in clinical use and provides deep insights into chondrocyte dedifferentiation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.