Evidence map›Paper›PMID 35477304›Full record

ArticlemSystems2022

Activation of the Type III Secretion System of Enteropathogenic Escherichia coli Leads to Remodeling of Its Membrane Composition and Function.

Anish Zacharia, Ritesh Ranjan Pal, Naama Katsowich, Chanchal Thomas Mannully, Aida Ibrahim, Sivan Alfandary, Raphael Serruya, Amit K Baidya, Sigal Ben-Yehuda, Ilan Rosenshine and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in mSystems, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Anish ZachariaThe Institute for Drug Research, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Ritesh Ranjan PalDepartment of Microbiology and Molecular Genetics, Institute of Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Naama KatsowichDepartment of Microbiology and Molecular Genetics, Institute of Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Chanchal Thomas MannullyThe Institute for Drug Research, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Aida IbrahimThe Institute for Drug Research, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Sivan AlfandaryThe Institute for Drug Research, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Raphael SerruyaThe Institute for Drug Research, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Amit K BaidyaDepartment of Microbiology and Molecular Genetics, Institute of Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Sigal Ben-YehudaDepartment of Microbiology and Molecular Genetics, Institute of Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Ilan RosenshineDepartment of Microbiology and Molecular Genetics, Institute of Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Arieh MoussaieffThe Institute for Drug Research, The Hebrew University of Jerusalemgrid.9619.7, Jerusalem, Israel.
Hebrew University of Jerusalem · ILIndian Association for the Cultivation of Science · INIndian Institute of Science Bangalore · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cell envelope of Gram-negative bacteria is a complex structure, essential for bacterial survival and for resistance to many antibiotics. Channels that cross the bacterial envelope and the host cell membrane form secretion systems that are activated upon attachment to host, enabling bacteria to inject effector molecules into the host cell, required for bacterium-host interaction. The type III secretion system (T3SS) is critical for the virulence of several pathogenic bacteria, including enteropathogenic Escherichia coli (EPEC). EPEC T3SS activation is associated with repression of carbon storage regulator (CsrA), resulting in gene expression remodeling, which is known to affect EPEC central carbon metabolism and contributes to the adaptation to a cell-adherent lifestyle in a poorly understood manner. We reasoned that the changes in the bacterial envelope upon attachment to the host and the activation of a secretion system may involve a modification of the lipid composition of bacterial envelope. Accordingly, we performed a lipidomics analysis on mutant strains that simulate T3SS activation. We saw a shift in glycerophospholipid metabolism toward the formation of lysophospholipids, attributed to corresponding upregulation of the phospholipase gene

Indexed as

Enteropathogenic Escherichia coliEscherichia coli ProteinsHumansLipidsRepressor ProteinsRNA-Binding ProteinsType III Secretion SystemsVancomycinCsrA protein, E coliEscherichia coli ProteinsLipidsRepressor ProteinsRNA-Binding ProteinsType III Secretion SystemsVancomycincell envelopeCesTCsrAenteropathogenic E. coliisoprenoidslipid metabolismlipidomicsphospholipidsterpenoidstype III secretion system

Identifiers

PMID35477304
PMCPMC9238428
OpenAlexW4224997233

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.