Evidence map›Paper›PMID 35476987›Full record

ArticleCell reports2022

circMbl functions in cis and in trans to regulate gene expression and physiology in a tissue-specific fashion.

Nagarjuna Reddy Pamudurti, Ines Lucia Patop, Aishwarya Krishnamoorthy, Osnat Bartok, Roni Maya, Noam Lerner, Reut Ashwall-Fluss, Jacob Vinay Vikas Konakondla, Tsevi Beatus, Sebastian Kadener

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.5field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 55 citations in OpenAlex.

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  4. The history and function of a circular RNA.Nature communications · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 2 countries.

Nagarjuna Reddy PamudurtiBiology Department, Brandeis University, Waltham, MA 02454, USA.
Ines Lucia PatopBiology Department, Brandeis University, Waltham, MA 02454, USA.
Aishwarya KrishnamoorthyBiology Department, Brandeis University, Waltham, MA 02454, USA.
Osnat BartokSilberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Roni MayaThe Rachel and Selim Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel; Department of Neurobiology, The Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Noam LernerThe Rachel and Selim Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel; Department of Neurobiology, The Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Reut Ashwall-FlussSilberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Jacob Vinay Vikas KonakondlaBiology Department, Brandeis University, Waltham, MA 02454, USA.
Tsevi BeatusThe Rachel and Selim Benin School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel; Department of Neurobiology, The Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel.
Sebastian KadenerBiology Department, Brandeis University, Waltham, MA 02454, USA; Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 9190401, Israel. Electronic address: skadener@brandeis.edu.
Hebrew University of Jerusalem · ILBrandeis University · US

Funding

Unraveling regulatory functions of circRNAs at the muscleblind locusR01GM124406 · NIGMS · BRANDEIS UNIVERSITY · PI Sebastian Kadener · 2017 to 2026
$2.4M
NIGMS NIH HHS R01 GM124406
6 · The paper itself

Abstract

Muscleblind (mbl) is an essential muscle and neuronal splicing regulator. Mbl hosts multiple circular RNAs (circRNAs), including circMbl, which is conserved from flies to humans. Here, we show that mbl-derived circRNAs are key regulators of MBL by cis- and trans-acting mechanisms. By generating fly lines to specifically modulate the levels of all mbl RNA isoforms, including circMbl, we demonstrate that the two major mbl protein isoforms, MBL-O/P and MBL-C, buffer their own levels by producing different types of circRNA isoforms in the eye and fly brain, respectively. Moreover, we show that circMbl has unique functions in trans, as knockdown of circMbl results in specific morphological and physiological phenotypes. In addition, depletion of MBL-C or circMbl results in opposite behavioral phenotypes, showing that they also regulate each other in trans. Together, our results illuminate key aspects of mbl regulation and uncover cis and trans functions of circMbl in vivo.

Indexed as

RNA, CircularRNA SplicingGene ExpressionNeuronsRNA, CircularcircMblcircRNACP: Molecular biologyDrosophilaMBNLmuscleblindRNA metabolismsplicing

Identifiers

PMID35476987
PMCPMC9352392
OpenAlexW4224881434

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.