Evidence map›Paper›PMID 35475784›Full record

ArticleParasite (Paris, France)2022

Molecular genetic diversity and bioinformatic analysis of Leucocytozoon sabrazesi based on the mitochondrial genes cytb, coxI and coxIII and co-infection of Plasmodium spp.

Pornpiroon Nooroong, Amaya Watthanadirek, Sutthida Minsakorn, Napassorn Poolsawat, Witchuta Junsiri, Nitipon Srionrod, Siriphan Sangchuai, Runglawan Chawengkirttikul, Panat Anuracpreeda

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 55% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Insights into the Biology ofMicroorganisms · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Pornpiroon NooroongParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Amaya WatthanadirekParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Sutthida MinsakornParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Napassorn PoolsawatParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Witchuta JunsiriParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Nitipon SrionrodParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Siriphan SangchuaiParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand - Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand.
Runglawan ChawengkirttikulDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Panat AnuracpreedaParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom 73170, Thailand.
Mahidol University · THSiriraj Hospital · TH

Funding

Mahidol University (Basic Research Fund: fiscal year 2021)Research Grant from the National Research Council of Thailand (NRCT) and the Mid-Career Research Grant co-founded by National Research Council of Thailand (NRCT) and Mahidol University NRCT5-RSA63015-23
6 · The paper itself

Abstract

Leucocytozoon sabrazesi is an intracellular haemoprotozoan parasite responsible for leucocytozoonosis, which is transmitted by insect vectors and affects chickens in tropical and subtropical areas in many countries. It causes huge economic losses due to decreased meat and egg production. In the present study, we used nested PCR to determine the genetic diversity of L. sabrazesi based on the cytb, coxI, coxIII and concatenated genes in chickens in Thailand. In addition, we found co-infections between L. sabrazesi and Plasmodium spp. (P. gallinaceum or P. juxtanucleare) in chickens that were not identified by microscopic examination of blood smears. The phylogenetic analysis indicated that L. sabrazesi cytb and coxIII genes were conserved with similarity ranging from 99.9 to 100% and 98 to 100%, respectively whereas the coxI gene was diverse, with similarities ranging from 97 to 100%. These findings ascertained the nucleotide analysis of the cytb, coxI, coxIII and concatenated sequences in which 4, 8, 10 and 9 haplotypes were found, respectively. In addition, it was found that the large number of synonymous substitutions and conservative amino acid replacements in these mitochondrial genes occurred by non-synonymous substitution. The evolutionary analysis of the K

Indexed as

CoinfectionHaemosporidaPlasmodiumAnimalsChickensComputational BiologyGenes, MitochondrialMolecular BiologyPhylogenyChickensCo-infectionGenetic diversityLeucocytozoon sabrazesiMitochondrial genesPlasmodium spp.Thailand

Identifiers

PMID35475784
PMCPMC9044968
OpenAlexW4225727864

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.