Evidence map›Paper›PMID 35475217›Full record

ArticleCell reports methods2021

Stable and reproducible homologous recombination enables CRISPR-based engineering in the fungus

Carlos Lax, María Isabel Navarro-Mendoza, Carlos Pérez-Arques, Eusebio Navarro, Francisco Esteban Nicolás, Victoriano Garre

Open access · goldAbstract read
In one paragraph

Article in Cell reports methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed
1.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 33 citations in OpenAlex.

  1. Article
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  4. The molecular basis of intrinsic resistance to azoles inAntimicrobial agents and chemotherapy · 2026
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  11. Molecular mechanisms that govern infection and antifungal resistance in Mucorales.Microbiology and molecular biology reviews : MMBR · 2024
    Review
  12. CRISPR/Cas9 improves targeted knock-in efficiency inBiotechnology notes (Amsterdam, Netherlands) · 2024
    Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Journal of fungi (Basel, Switzerland) · 2023
    Review
  18. Heterochromatin and RNAi act independently to ensure genome stability in Mucorales human fungal pathogens.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  19. Mucormycosis in 2023: an update on pathogenesis and management.Frontiers in cellular and infection microbiology · 2023
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Carlos LaxDepartamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain.
María Isabel Navarro-MendozaDepartment of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
Carlos Pérez-ArquesDepartment of Molecular Genetics and Microbiology, Duke University Medical Center, Durham, NC 27710, USA.
Eusebio NavarroDepartamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain.
Francisco Esteban NicolásDepartamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain.
Victoriano GarreDepartamento de Genética y Microbiología, Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain.
Universidad de Murcia · ESDuke Medical Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mucormycosis is a lethal and emerging disease that has lacked a genetic model fulfilling both high virulence and the possibility of performing stable and reproducible gene manipulation by homologous recombination (HR). Here, we developed a new methodology to successfully perform HR in

Indexed as

MucormycosisRhizopusAnimalsHomologous RecombinationMiceUracilUracilcrgACRISPRgenetic modelhomologous recombinationleuAMucoralesmucormycosispyrFRhizopus microsporusvirulence

Identifiers

PMID35475217
PMCPMC9017206
OpenAlexW4200365289

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.