ArticleCell reports methods2021
GEM-IL: A highly responsive fluorescent lactate indicator.
Article in Cell reports methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- A red fluorescent genetically encoded biosensor for in vivo imaging of extracellular L-lactate dynamics.Nature communications · 2025Article
- Synthesis and application of a photocaged-L-lactate for studying the biological roles of L-lactate.Communications chemistry · 2025Article
- Illuminating anions in biology with genetically encoded fluorescent biosensors.Current opinion in chemical biology · 2025Review
- Elucidating the spatiotemporal dynamics of glucose metabolism with genetically encoded fluorescent biosensors.Cell reports methods · 2024Review
- Comprehensive multiscale analysis of lactate metabolic dynamics in vitro and in vivo using highly responsive biosensors.Nature protocols · 2024Article
- Developing inhibitory peptides against SARS-CoV-2 envelope protein.PLoS biology · 2024Article
- High-Performance Genetically Encoded Green Fluorescent Biosensors for Intracellular l-Lactate.ACS central science · 2024Article
- Mitochondrial ATP concentration decreases immediately after glucose administration to glucose-deprived hepatocytes.FEBS open bio · 2024Article
- Lactate biosensors for spectrally and spatially multiplexed fluorescence imaging.Nature communications · 2023Article
- Modern Microscopic Approaches to Astrocytes.International journal of molecular sciences · 2023Review
- Illuminating lactate in cells, mice, and patient samples.Cell metabolism · 2023Article
- Ultrasensitive sensors reveal the spatiotemporal landscape of lactate metabolism in physiology and disease.Cell metabolism · 2023Article
- A Selective Fluorescent l-Lactate Biosensor Based on an l-Lactate-Specific Transcription Regulator and Förster Resonance Energy Transfer.Biosensors · 2022Article
- A high-throughput multiparameter screen for accelerated development and optimization of soluble genetically encoded fluorescent biosensors.Nature communications · 2022Article
Corrections and comments
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Authors and funding
18 authors at 3 institutions in 3 countries.
Funding
Abstract
Lactate metabolism has been shown to have increasingly important implications in cellular functions as well as in the development and pathophysiology of disease. The various roles as a signaling molecule and metabolite have led to interest in establishing a new method to detect lactate changes in live cells. Here we report our development of a genetically encoded metabolic indicator specifically for probing lactate (GEM-IL) based on superfolder fluorescent proteins and mutagenesis. With improvements in its design, specificity, and sensitivity, GEM-IL allows new applications compared with the previous lactate indicators, Laconic and Green Lindoblum. We demonstrate the functionality of GEM-IL to detect differences in lactate changes in human oncogenic neural progenitor cells and mouse primary ventricular myocytes. The development and application of GEM-IL show promise for enhancing our understanding of lactate dynamics and roles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.