ArticleCell reports methods2021
An integrated pipeline for mammalian genetic screening.
Article in Cell reports methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Computational blueprints for cell fate programming.Stem cell reports · 2026Review
- Endocrinology at a Miniature Level: Pluripotent Stem-Cell-Derived Organoid Models of Hypothalamus-Pituitary Axes.Biomolecules · 2026Review
- Branched Schrödinger Bridge Matching.ArXiv · 2026Article
- Entangled Schrödinger Bridge Matching.ArXiv · 2025Article
- Initiation of meiosis from human iPSCs under defined conditions through identification of regulatory factors.Science advances · 2025Article
- A Massively ParallelACS synthetic biology · 2024Article
- Creation of de novo cryptic splicing for ALS and FTD precision medicine.Science (New York, N.Y.) · 2024Article
- Induction of Meiosis from Human Pluripotent Stem Cells.bioRxiv : the preprint server for biology · 2024Article
- Mammalian cell growth characterisation by a non-invasive plate reader assay.Nature communications · 2024Article
- Creation of de novo cryptic splicing for ALS/FTD precision medicine.bioRxiv : the preprint server for biology · 2023Article
- Article
- Progeria and Aging-Omics Based Comparative Analysis.Biomedicines · 2022Article
- MegaGate: A toxin-less gateway molecular cloning tool.STAR protocols · 2021Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the recent advancements in genome editing, next-generation sequencing (NGS), and scalable cloning techniques, scientists can now conduct genetic screens at unprecedented levels of scale and precision. With such a multitude of technologies, there is a need for a simple yet comprehensive pipeline to enable systematic mammalian genetic screening. In this study, we develop unique algorithms for target identification and a toxin-less Gateway cloning tool, termed MegaGate, for library cloning which, when combined with existing genetic perturbation methods and NGS-coupled readouts, enable versatile engineering of relevant mammalian cell lines. Our integrated pipeline for sequencing-based target ascertainment and modular perturbation screening (STAMPScreen) can thus be utilized for a host of cell state engineering applications.
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