ArticleCell reports methods2021
Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH.
Article in Cell reports methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Simultaneous spatial transcriptomics and morphology profiling as tools to explore how microglia change with age.Nature aging · 2026Article
- Interplay between microtubule interactome, myonuclei mechanotransduction, and positioning in myopathies.Nucleus (Austin, Tex.) · 2025Review
- Article
- EASI-ORC: A pipeline for the efficient analysis and segmentation of smFISH images for organelle-RNA colocalization measurements in yeast.Communications biology · 2025Article
- GP-HTNLoc: A graph prototype head-tail network-based model for multi-label subcellular localization prediction of ncRNAs.Computational and structural biotechnology journal · 2024Article
- Spatial omics advances for in situ RNA biology.Molecular cell · 2024Review
- High-throughput image processing software for the study of nuclear architecture and gene expression.Scientific reports · 2024Article
- Quantifying spatial dynamics of Mycobacterium tuberculosis infection of human macrophages using microfabricated patterns.Cell reports methods · 2023Article
- Technical Advances and Applications of Spatial Transcriptomics.GEN biotechnology · 2023Article
- Diversity of cells and signals in the cardiovascular system.The Journal of physiology · 2023Article
- Article
- FISH-quant v2: a scalable and modular tool for smFISH image analysis.RNA (New York, N.Y.) · 2022Article
- DeepSpot: A deep neural network for RNA spot enhancement in single-molecule fluorescence in-situ hybridization microscopy images.Biological imaging · 2022Article
- EL-RMLocNet: An explainable LSTM network for RNA-associated multi-compartment localization prediction.Computational and structural biotechnology journal · 2022Article
- Cellular Polarity Transmission to the Nucleus.Results and problems in cell differentiation · 2022Article
- Prediction of RNA subcellular localization: Learning from heterogeneous data sources.iScience · 2021Review
Corrections and comments
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in single-cell RNA sequencing have allowed for the identification of cellular subtypes on the basis of quantification of the number of transcripts in each cell. However, cells might also differ in the spatial distribution of molecules, including RNAs. Here, we present DypFISH, an approach to quantitatively investigate the subcellular localization of RNA and protein. We introduce a range of analytical techniques to interrogate single-molecule RNA fluorescence
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Registered trials
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