ArticleScientific reports2022
Impact of immobilization strategies on the activity and recyclability of lipases in nanomagnetic supports.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 50 citations in OpenAlex.
- PGPR-Treated Spent Mushroom Substrate Enhances Lignocellulose Degradation, Enzyme Activities, and Microbial Restructuring to Sustain Blueberry Rhizosphere Fertility.Microorganisms · 2026Article
- Immobilization of TtAlaDH on magnetic nanoparticles: effects on stability and reuse.Applied microbiology and biotechnology · 2026Article
- Impact of functionalization linker chemistry on cellulase immobilization: a comparative study of TEOS, APTS, and MPTS functionalized magnetic nanoparticles.RSC advances · 2026Article
- Solvent-Free Catalytic Synthesis of Ethyl Butyrate Using Immobilized Lipase Based on Hydrophobically Functionalized Dendritic Fibrous Nano-Silica.Foods (Basel, Switzerland) · 2025Article
- Optimization and characterization of a lactate-oxidase electrode.RSC advances · 2025Article
- Catalytically active inclusion bodies of cold-adapted lipase: production and its industrial potential.AMB Express · 2025Article
- Article
- Immobilized inclusion bodies of recombinant cold-adaptive lipase from Antarctic Pseudomonas sp. as catalysts.Applied microbiology and biotechnology · 2025Article
- Enhanced activity and self-regeneration in dynameric cross-linked enzyme nanoaggregates.Science advances · 2025Article
- Biofunctionalization of Magneto-Plasmonic FeMolecules (Basel, Switzerland) · 2025Article
- Carboxylated nanocellulose from quinoa husk for enhanced protease immobilization and stability of protease in biotechnological applications.Scientific reports · 2025Article
- Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes.Applied biochemistry and biotechnology · 2024Review
- Co-administration of xylo-oligosaccharides produced by immobilized Aspergillus terreus xylanase with carbimazole to mitigate its adverse effects on the adrenal gland.Scientific reports · 2024Article
- Production of a recyclable nanobiocatalyst to synthesize quinazolinone derivatives.RSC advances · 2022Article
- The synergism of lytic polysaccharide monooxygenases with lichenase and their co-immobilization on silica nanospheres for green conversion of lichen biomass.Frontiers in nutrition · 2022Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of enzymes immobilized on nanomagnetic supports has produced surprising results in catalysis, mainly due to the increase in surface area and the potential for recovery and reuse. However, the meticulous control of the process and difficulties in reproducibility have made industrial-scale applications unfeasible. Furthermore, the role of conjugation strategies in the catalytic activity and recycling of catalysts is unclear. Therefore, the objective of this study was to compare the conjugation of enzymes on nanomagnetic supports through physical adsorption (naked) or covalent bonding with mercaptopropyltrimethoxysilane (MPTS) and aminopropyltriethoxysilane (APTS) ligands. The free lipase obtained from Rhizomucor miehei was used as a model enzyme. Total protein and enzyme activity were determined using spectrophotometry (UV-Vis) and the p-nitrophenyl palmitate (p-NPP) hydrolysis method. The results indicated that a more significant enzyme surface loading does not always mean better immobilization success. The physical adsorption binding strategy had higher surface loading and low catalytic activity. On the other hand, covalent coupling with free NH2 had an excellent catalytic activity with very low surface loading. Finally, we show that recyclability can be improved with conjugation mediated by disulfide bonds. The findings presented here are essential for developing nanoconjugates with high enzymatic activity, which can guarantee the success of several industrial applications.
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