ArticleEnvironmental science & technology2022
Single-Particle Resolution Fluorescence Microscopy of Nanoplastics.
Article in Environmental science & technology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 75 citations in OpenAlex.
- Polarization Holographic 4D Phenotyping Reveals Nanoplastic Induced Dysfunction in Zooplankton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Fluorescent Labeling Strategies for Tracking Micro- and Nanoplastics in Biological Systems.Materials (Basel, Switzerland) · 2026Review
- Fluorescent nanoplastics: What steps are needed towards a representative toolkit?Microplastics and nanoplastics · 2026Review
- Fluorescently Labelled Polypropylene as a Model Microplastic for Cellular Imaging.Polymer chemistry · 2026Article
- Biodistribution of nanoplastics in mice: advancing analytical techniques using metal-doped plastics.Communications biology · 2025Article
- Quantifying Nanoplastic Toxicity Using Gold-Core Polystyrene Nanoparticles: In vivo Evaluation and Human Risk Extrapolation.International journal of nanomedicine · 2025Article
- New Fluorophore and Its Applications in Visualizing Polystyrene Nanoplastics in Bean Sprouts and HeLa Cells.Molecules (Basel, Switzerland) · 2023Article
- Nanoplastic production procedure for scientific purposes: PP, PVC, PE-LD, PE-HD, and PS.Heliyon · 2023Article
- Hazard Assessment of Polystyrene Nanoplastics in Primary Human Nasal Epithelial Cells, Focusing on the Autophagic Effects.Biomolecules · 2023Article
- Spectro-Microscopic Techniques for Studying Nanoplastics in the Environment and in Organisms.Angewandte Chemie (International ed. in English) · 2023Review
- Regulatory landscape of nanotechnology and nanoplastics from a global perspective.Regulatory toxicology and pharmacology : RTP · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Understanding of nanoplastic prevalence and toxicology is limited by imaging challenges resulting from their small size. Fluorescence microscopy is widely applied to track and identify microplastics in laboratory studies and environmental samples. However, conventional fluorescence microscopy, due to diffraction, lacks the resolution to precisely localize nanoplastics in tissues, distinguish them from free dye, or quantify them in environmental samples. To address these limitations, we developed techniques to label nanoplastics for imaging with stimulated emission depletion (STED) microscopy to achieve resolution at an order of magnitude superior to conventional fluorescence microscopy. These techniques include (1) passive sorption; (2) swell incorporation; and (3) covalent coupling of STED-compatible fluorescence dyes to nanoplastics. We demonstrate that our labeling techniques, combined with STED microscopy, can be used to resolve nanoplastics of different shapes and compositions as small as 50 nm. The longevity of dye labeling is demonstrated in different media and conditions of biological and environmental relevance. We also test STED imaging of nanoplastics in exposure experiments with the model worm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.