ArticleBiology of reproduction2022
Biochemical and molecular characterization of sialylated cervical mucins in sheep†.
Article in Biology of reproduction, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Soybean Oligosaccharides Mitigate HFD-Induced Obesity in Mice with Changes in the Gut Mucus-Microbiota Axis.Nutrients · 2026Article
- Towards cervical AI of sheep with stored semen: latest insights and future developments.The Journal of reproduction and development · 2026Review
- Seminal plasma protects frozen-thawed ram spermatozoa from neutrophil attack: a complement mediated dynamic.Reproduction (Cambridge, England) · 2025Article
- The cervical microbiome of ewe breeds with known divergent fertility following artificial insemination with frozen-thawed semen.Scientific reports · 2025Article
- Optimizing artificial insemination in goats: semen deposition site and vaginal mucus characteristics as predictive biomarkers.Frontiers in veterinary science · 2025Article
- Genome-wide association and functional annotation analyses reveal candidate genes and pathways associated with various ewe longevity indicators in U.S. Katahdin sheep.Frontiers in genetics · 2025Article
- Structural variant landscapes reveal convergent signatures of evolution in sheep and goats.Genome biology · 2024Article
- Expression Analysis of Genes Corresponding to Mucins and Their Glycans from Cervical Tissue Using RNA Sequencing.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Metabolic signature of cervical mucus in ewe breeds with divergent cervical sperm transport: a focus on metabolites involved in amino acid metabolism.Metabolomics : Official journal of the Metabolomic Society · 2023Article
- Uterus proliferative period ceRNA network of Yunshang black goat reveals candidate genes on different kidding number trait.Frontiers in endocrinology · 2023Article
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sialic acid occupies terminal positions on O-glycans of cervical mucins, where they contribute to the increased viscosity of mucin thereby regulating sperm transport. This study characterized the sialylated cervical mucins from follicular phase mucus of six European ewe breeds with known differences in pregnancy rates following cervical artificial insemination (AI) using frozen-thawed semen at both synchronized and natural estrus cycles. These were Suffolk (low fertility) and Belclare (medium fertility) in Ireland, Ile de France and Romanov (both with medium fertility) in France, and Norwegian White Sheep (NWS) and Fur (both with high fertility) in Norway. Expression of mucin and sialic acid related genes was quantified using RNA-sequencing in cervical tissue from Suffolk, Belclare, Fur, and NWS only. Cervical tissue was also assessed for the percentage of cervical epithelial populated by mucin secreting goblet cells in the same four ewe breeds. Biochemical analysis showed that there was an effect of ewe breed on sialic acid species, which was represented by Suffolk having higher levels of Neu5,9Ac2 compared with NWS (P < 0.05). Suffolk ewes had a lower percentage of goblet cells than Fur and NWS (P < 0.05). Gene expression analysis identified higher expression of MUC5AC, MUC5B, ST6GAL1, and ST6GAL2 and lower expression of ST3GAL3, ST3GAL4, and SIGLEC10 in Suffolk compared with high fertility ewe breeds (P < 0.05). Our results indicate that specific alterations in sialylated mucin composition may be related to impaired cervical sperm transport.
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