Evidence map›Paper›PMID 35468928›Full record

ArticleScientific reports2022

The macroscopic limit to synchronization of cellular clocks in single cells of Neurospora crassa.

Jia Hwei Cheong, Xiao Qiu, Yang Liu, Ahmad Al-Omari, James Griffith, Heinz-Bernd Schüttler, Leidong Mao, Jonathan Arnold

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Origin of the clock inFrontiers in molecular biosciences · 2025
    Review
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Jia Hwei Cheong *Chemistry Department, University of Georgia, Athens, GA, 30602, USA.
Xiao Qiu *Institute of Bioinformatics, University of Georgia, Athens, GA, 30602, USA.
Yang LiuChemistry Department, University of Georgia, Athens, GA, 30602, USA.
Ahmad Al-OmariDepartment of Biomedical Systems and Informatics Engineering, Yarmouk University, Irbid, 21163, Jordan.
James GriffithGenetics Department, University of Georgia, Athens, GA, 30602, USA.
Heinz-Bernd SchüttlerDepartment of Physics and Astronomy, University of Georgia, Athens, GA, 30602, USA.
Leidong MaoSchool of Electrical and Computer Engineering, College of Engineering, University of Georgia, Athens, GA, 30602, USA. mao@uga.edu.
Jonathan ArnoldGenetics Department, University of Georgia, Athens, GA, 30602, USA. arnold@uga.edu.
University of Georgia · USYarmouk University · JO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We determined the macroscopic limit for phase synchronization of cellular clocks in an artificial tissue created by a "big chamber" microfluidic device to be about 150,000 cells or less. The dimensions of the microfluidic chamber allowed us to calculate an upper limit on the radius of a hypothesized quorum sensing signal molecule of 13.05 nm using a diffusion approximation for signal travel within the device. The use of a second microwell microfluidic device allowed the refinement of the macroscopic limit to a cell density of 2166 cells per fixed area of the device for phase synchronization. The measurement of averages over single cell trajectories in the microwell device supported a deterministic quorum sensing model identified by ensemble methods for clock phase synchronization. A strong inference framework was used to test the communication mechanism in phase synchronization of quorum sensing versus cell-to-cell contact, suggesting support for quorum sensing. Further evidence came from showing phase synchronization was density-dependent.

Indexed as

Neurospora crassaDiffusionLab-On-A-Chip DevicesQuorum Sensing

Identifiers

PMID35468928
PMCPMC9039089
OpenAlexW4225260683

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.