ArticleClinical and experimental dermatology2022
Effect of the most common wound antiseptics on human skin fibroblasts.
Article in Clinical and experimental dermatology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Effectiveness of Silk Fibroin Dressing Compared to Saline Dressing on Wound Healing in Post-Laparotomy Patients with Superficial Surgical Site Infections:Sultan Qaboos University medical journal · 2025Trial
- Cold Atmospheric Plasma Disrupts Microbial Wound Bioburden: In Vitro, Porcine MRSA Biofilm, and Clinical Fluorescence Evidence from Bench to Bedside.Biomedicines · 2026Article
- Preoperative penoscrotal swab microbiology and postoperative infection concordance in malleable penile prosthesis surgery: A prospective randomized study of skin antisepsis.International journal of impotence research · 2026Article
- Oxygen and ROS Delivery for Infected Wound Healing and Future Prospects.Advanced healthcare materials · 2026Review
- Stem Cell and Exosome Therapy in Wound Healing: Traps, Paradoxes, and Tricks Transforming Paradigms.Biomedicines · 2025Article
- Evaluation of the Therapeutic Efficacy of the Newly Formulated Drug "Novostron" as an Experimental Basis for Clinical Trials.ACS omega · 2025Article
- Antimicrobial Efficacy of Impregnated Human Acellular Dermal Substitutes in Burn Wound Models.Antibiotics (Basel, Switzerland) · 2025Article
- New Insights Regarding the Use of Relevant Synthetic Compounds in Dentistry.Molecules (Basel, Switzerland) · 2024Review
- Sustainable Primary Cell Banking for Topical Compound Cytotoxicity Assays: Protocol Validation on Novel Biocides and Antifungals for Optimized Burn Wound Care.European burn journal · 2024Article
- Tolerability of N-chlorotaurine in comparison with routinely used antiseptics: an in vitro study on chondrocytes.Pharmacological reports : PR · 2024Article
- Article
- Pressure-Spun Fibrous Surgical Sutures for Localized Antibacterial Delivery: Development, Characterization, and In Vitro Evaluation.ACS applied materials & interfaces · 2023Article
- Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone-Iodine and Chlorhexidine in Mono and Coculture Models.Biomedicines · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAntiseptics are used for the cleansing of acute or chronic wounds to eliminate micro-organisms from the wound bed. However, they have effects on the skin cells.
aimTo determine the effects of hexetidine, povidone-iodine (PI), undecylenamidopropyl-betaine/polyhexanide (UBP), chlorhexidine, disodium eosin and hydrogen peroxide on human skin fibroblasts.
methodsCCD-1064Sk cells were treated with hexetidine, PI, UBP, chlorhexidine, disodium eosin or hydrogen peroxide. Spectrophotometry was used to measure cell viability and flow cytometry was used to study apoptosis and necrosis after the treatment. In vitro wound scratch assays were performed to determine the gap closure.
resultsAll antiseptics significantly reduced the viability of human skin fibroblasts compared with controls. The percentage wound closure was lower with hexetidine, PI and UBP. The scratch assay could not be measured after treatments with chlorhexidine, disodium eosin or hydrogen peroxide, owing to their cytotoxicity. The apoptosis/necrosis experiments evidenced a significant reduction in viable cells compared with controls. An increased percentage of apoptotic cells was observed after treatment with all antiseptics. Compared with controls, the percentage of necrotic cells was significantly increased with all antiseptics except for hexetidine.
conclusionThe proliferation, migration and viability of human skin fibroblasts are reduced by treatment with hexetidine, PI, UBP, chlorhexidine, disodium eosin and hydrogen peroxide.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.