ArticleThe Journal of antimicrobial chemotherapy2022
Antimicrobial resistance genes aph(3')-III, erm(B), sul2 and tet(W) abundance in animal faeces, meat, production environments and human faeces in Europe.
Article in The Journal of antimicrobial chemotherapy, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Metagenomic analysis reveals resistome characteristics and high-risk resistance genes in the pig nasal cavities, feces, and farm dust.Animal microbiome · 2026Article
- KEGG orthology-based machine learning reveals functional determinants of antimicrobial resistance inMicrobiology spectrum · 2026Article
- Mind the governance gap: a one health scoping review of national AMR performance indicators.npj antimicrobials and resistance · 2026Article
- Coccidiosis prevention strategies shape the microbiome, resistome and mobilome composition in the broiler gut.Animal microbiome · 2026Article
- Animal type outweighs growth stage in shaping the active antibiotic resistome in livestock manure.Frontiers in microbiology · 2026Article
- One Health, One Microbiome.Microbiome · 2025Review
- Bacterial Antimicrobial Resistance in Meat Products-Current Concepts.Foods (Basel, Switzerland) · 2025Review
- Antimicrobial treatment affects the microbiome and resistome of both treated and untreated rehabilitating harbour seals (Phoca vitulina).Animal microbiome · 2025Article
- Article
- City-scale monitoring of antibiotic resistance genes by digital PCR and metagenomics.Environmental microbiome · 2024Article
- Genotypic Antimicrobial Resistance Profiles of DiarrheagenicInfection and drug resistance · 2024Article
- The Use of Antibiotics and Antimicrobial Resistance in Veterinary Medicine, a Complex Phenomenon: A Narrative Review.Antibiotics (Basel, Switzerland) · 2023Review
- Recent Trends of Antibiotic Resistance inAntibiotics (Basel, Switzerland) · 2023Article
- Detection of Acquired Antibiotic Resistance Genes in Domestic Pig (Antibiotics (Basel, Switzerland) · 2022Article
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Authors and funding
21 authors.
Funding
Abstract
backgroundReal-time quantitative PCR (qPCR) is an affordable method to quantify antimicrobial resistance gene (ARG) targets, allowing comparisons of ARG abundance along animal production chains.
objectivesWe present a comparison of ARG abundance across various animal species, production environments and humans in Europe. AMR variation sources were quantified. The correlation of ARG abundance between qPCR data and previously published metagenomic data was assessed.
methodsA cross-sectional study was conducted in nine European countries, comprising 9572 samples. qPCR was used to quantify abundance of ARGs [aph(3')-III, erm(B), sul2, tet(W)] and 16S rRNA. Variance component analysis was conducted to explore AMR variation sources. Spearman's rank correlation of ARG abundance values was evaluated between pooled qPCR data and earlier published pooled metagenomic data.
resultsARG abundance varied strongly among animal species, environments and humans. This variation was dominated by between-farm variation (pigs) or within-farm variation (broilers, veal calves and turkeys). A decrease in ARG abundance along pig and broiler production chains ('farm to fork') was observed. ARG abundance was higher in farmers than in slaughterhouse workers, and lowest in control subjects. ARG abundance showed a high correlation (Spearman's ρ > 0.7) between qPCR data and metagenomic data of pooled samples.
conclusionsqPCR analysis is a valuable tool to assess ARG abundance in a large collection of livestock-associated samples. The between-country and between-farm variation of ARG abundance could partially be explained by antimicrobial use and farm biosecurity levels. ARG abundance in human faeces was related to livestock antimicrobial resistance exposure.
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