ArticlePhotoacoustics2022
Label-free analytic histology of carotid atherosclerosis by mid-infrared optoacoustic microscopy.
Article in Photoacoustics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Cell apoptosis in ischemic stroke: Focus on lipid metabolism.Neural regeneration research · 2026Article
- Effective point spread function modeling of photoacoustic remote sensing microscopy towards resolution enhancement.Photoacoustics · 2026Article
- Single-Cell Metabolic Imaging and Digital Scoring of Fat Tissue Remodeling by Label-Free Metabolic Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Revealing hidden tissue architecture with mid-infrared dichroism photoacoustic microscopy.Light, science & applications · 2026Article
- Label-free mid-infrared dichroism-sensitive photoacoustic microscopy for histostructural analysis of engineered heart tissues.Light, science & applications · 2026Article
- A comprehensive review of high-performance photoacoustic microscopy systems.Photoacoustics · 2025Review
- A joint photoacoustic imaging and broadband spectral analysis for early-stage intraoperative pathology assessment: A case study with colorectal cancer.Photoacoustics · 2025Article
- Label-free optical microscopy with artificial intelligence: a new paradigm in pathology.Biophotonics discovery · 2025Review
- Unsupervised inter-domain transformation for virtually stained high-resolution mid-infrared photoacoustic microscopy using explainable deep learning.Nature communications · 2024Article
- Mid-infrared photoacoustic brain imaging enabled by cascaded gas-filled hollow-core fiber lasers.Neurophotonics · 2024Article
- Deep learning-based virtual staining, segmentation, and classification in label-free photoacoustic histology of human specimens.Light, science & applications · 2024Article
- Tumor-specific enhanced NIR-II photoacoustic imaging via photothermal and low-pH coactivated AuNR@PNIPAM-VAA nanogel.Journal of nanobiotechnology · 2024Article
- Azimuth mapping of fibrous tissue in linear dichroism-sensitive photoacoustic microscopy.Photoacoustics · 2023Article
- Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy.Npj imaging · 2023Article
- Optoacoustic biomarkers of lipids, hemorrhage and inflammation in carotid atherosclerosis.Frontiers in cardiovascular medicine · 2023Review
- In vivo quantitative photoacoustic evaluation of the liver and kidney pathology in tyrosinemia.Photoacoustics · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background and aims: Analysis of atherosclerotic plaque composition is a vital tool for unraveling the pathological metabolic processes that contribute to plaque growth. Methods: We visualize the constitution of human carotid plaques by mid-infrared optoacoustic microscopy (MiROM), a method for label-free analytic histology that requires minimal tissue preparation, rapidly yielding large field-of-view en-face images with a resolution of a few micrometers. We imaged endarterectomy specimens (n = 3, 12 sections total) at specific vibrational modes, targeting carbohydrates, lipids and proteins. Additionally, we recorded spectra at selected tissue locations. We identified correlations in the variability in this high-dimensional data set using non-negative matrix factorization (NMF). Results: We visualized high-risk plaque features with molecular assignment. Consistent NMF components relate to different dominant tissue constituents, dominated by lipids, proteins, and cholesterol and carbohydrates respectively. Conclusions: These results introduce MiROM as an innovative, stain-free, analytic histology technology for the biochemical characterization of complex human vascular pathology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.