ArticleFrontiers in bioengineering and biotechnology2022
Rapid and Facile Preparation of Giant Vesicles by the Droplet Transfer Method for Artificial Cell Construction.
Article in Frontiers in bioengineering and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- QuantGUV: Quantifying Encapsulation Efficiency of Small Molecules in Giant Unilamellar Vesicles.ACS applied materials & interfaces · 2026Article
- Bottom-Up Synthetic Biology for Artificial Cell Design: From Scaffold Materials to Functional Integration.Journal of microbiology and biotechnology · 2026Review
- Optimizing spatial organization of FtsZ rings for large-scale constriction in synthetic cells.Nature communications · 2026Article
- Functional reconstitution of bacterial ESCRT-III protein PspA identifies key regions in membrane binding and remodeling.Protein science : a publication of the Protein Society · 2025Article
- DNA Flipping as Facile Mechanism for Transmembrane Signaling in Synthetic Cells.Journal of the American Chemical Society · 2025Article
- Lipid Modification and Membrane Localization of Proteins in Cell-Free System.ACS synthetic biology · 2025Article
- Nuclear Assembly in Giant Unilamellar Vesicles Encapsulating Xenopus Egg Extract.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Rapid Multi-Well Evaluation of Assorted Materials for Hydrogel-Assisted Giant Unilamellar Vesicle Production: Empowering Bottom-Up Synthetic Biology.Gels (Basel, Switzerland) · 2025Article
- Dimensions, stability, and deformability of DOPC-cholesterol giant unilamellar vesicles formed by droplet transfer.Open research Europe · 2025Article
- Interdisciplinary investigations into the biophysics of the origins of life.Biophysics and physicobiology · 2025Article
- The Potential of Artificial Cells Functioning underACS synthetic biology · 2024Article
- Formation of Giant Unilamellar Vesicles Assisted by Fluorinated Nanoparticles.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Regulated N-Terminal Modification of Proteins Synthesized Using a Reconstituted Cell-Free Protein Synthesis System.ACS synthetic biology · 2023Article
- Article
- A Practical Guide to Preparation and Applications of Giant Unilamellar Vesicles Formed via Centrifugation of Water-in-Oil Emulsion Droplets.Membranes · 2023Review
- Cell-free expression of RuBisCO for ATP production in the synthetic cells.Synthetic biology (Oxford, England) · 2023Article
- Light-Switchable Membrane Permeability in Giant Unilamellar Vesicles.Pharmaceutics · 2022Article
- Phospholipid synthesis inside phospholipid membrane vesicles.Communications biology · 2022Article
- Commentary: Rapid and facile preparation of giant vesicles by the droplet transfer method for artificial cell construction.Frontiers in bioengineering and biotechnology · 2022Article
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2 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Giant vesicles have been widely used for the bottom-up construction of artificial (or synthetic) cells and the physicochemical analysis of lipid membranes. Although methods for the formation of giant vesicles and the encapsulation of molecules within them have been established, a standardized protocol has not been shared among researchers including non-experts. Here we proposed a rapid and facile protocol that allows the formation of giant vesicles within 30 min. The quality of the giant vesicles encapsulating a cell-free protein expression system was comparable to that of the ones formed using a conventional method, in terms of the synthesis of both soluble and membrane proteins. We also performed protein synthesis in artificial cells using a lyophilized cell-free mixture and showed an equivalent level of protein synthesis. Our method could become a standard method for giant vesicle formation suited for artificial cell research.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.