ArticleFood science and biotechnology2022
Development of a multiplex PCR assay for the simultaneous detection of big blue octopus (
Article in Food science and biotechnology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- A Multiplex PCR-Based Assay for Authentication of Six Commercially Important Cephalopod Species.Foods (Basel, Switzerland) · 2026Article
- Detection of Certain Berries in Difficult Samples by Singleplex and Multiplex Real-Time PCR-HRM: A Case Study of Pitfalls.Methods and protocols · 2026Article
- Development and intralaboratory validation of threeFood science and biotechnology · 2023Article
- Filling Analytical Gaps in Allergen Detection─Real-Time PCR for the Detection of Commercially Relevant Cephalopods and Gastropods in Food.Journal of agricultural and food chemistry · 2023Article
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Authors and funding
3 authors.
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Abstract
Since octopuses are similar in appearance and can be processed into various forms, seafood fraud has been reported. In this study, we developed the PCR assay to simultaneously detect three octopuses (big blue octopus, giant Pacific octopus, and common octopus). Specific primer sets were designed based on COI gene. We observed that the specific PCR amplicon sizes were 84 bp for big blue octopus, 117 bp for giant Pacific octopus, and 166 bp for common octopus, respectively. This assay was then used to test for specificity and did not show cross-reactivity with 15 cephalopods families. The limit of detection of the multiplex PCR assay was 0.1 pg. Subsequently, 30 commercial food products were then monitored to evaluate the applicability of this assay. All products were specifically amplified, and three octopus species of interest were distinguished. Therefore, this assay can be used as an octopus authentication tool in the seafood industry.
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