Evidence map›Paper›PMID 35463467›Full record

ArticleSTAR protocols2022

High-yield overproduction and purification of human aquaporins from

Tamim Al-Jubair, Jonas Hyld Steffen, Julie Winkel Missel, Philip Kitchen, Mootaz M Salman, Roslyn M Bill, Pontus Gourdon, Susanna Törnroth-Horsefield

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. International journal of molecular sciences · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 3 countries.

Tamim Al-JubairDepartment of Biochemistry and Structural Biology, Lund University, P.O. Box 124, 221 00 Lund, Sweden.
Jonas Hyld SteffenDepartment of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Julie Winkel MisselDepartment of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Philip KitchenCollege of Health and Life Sciences, Aston University, Aston Triangle, B4 7ET Birmingham, UK.
Mootaz M SalmanDepartment of Physiology, Anatomy and Genetics, Kavli Institute for NanoScience Discovery, University of Oxford, Parks Road, OX1 3PT Oxford, UK.
Roslyn M BillCollege of Health and Life Sciences, Aston University, Aston Triangle, B4 7ET Birmingham, UK.
Pontus GourdonDepartment of Biomedical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Susanna Törnroth-HorsefieldDepartment of Biochemistry and Structural Biology, Lund University, P.O. Box 124, 221 00 Lund, Sweden.
University of Copenhagen · DKAston University · GBLund University · SEParkinson's UK · GB

Funding

Biotechnology and Biological Sciences Research Council BB/P025927/1
6 · The paper itself

Abstract

Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast

Indexed as

AquaporinsSaccharomycetalesHumansPichiaSaccharomyces cerevisiaeAquaporinsCell MembraneMolecular BiologyProtein BiochemistryProtein expression and purification

Identifiers

PMID35463467
PMCPMC9026565
OpenAlexW4223526669

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.