ArticleThe Journal of cell biology1987
Microtubule dynamics in vivo: a test of mechanisms of turnover.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers.Journal of visualized experiments : JoVE · 2017Article
- Synergistic suppression of noscapine and conventional chemotherapeutics on human glioblastoma cell growth.Acta pharmacologica Sinica · 2013Article
- Paracrine and epigenetic control of trophectoderm differentiation from human embryonic stem cells: the role of bone morphogenic protein 4 and histone deacetylases.Stem cells and development · 2011Article
- Cytoplasmic linker proteins promote microtubule rescue in vivo.The Journal of cell biology · 2002Article
- Antagonistic forces generated by myosin II and cytoplasmic dynein regulate microtubule turnover, movement, and organization in interphase cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Region-specific microtubule transport in motile cells.The Journal of cell biology · 2000Article
- Stepwise reconstitution of interphase microtubule dynamics in permeabilized cells and comparison to dynamic mechanisms in intact cells.The Journal of cell biology · 1998Article
- Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling.The Journal of cell biology · 1997Article
- Microtubule release from the centrosome.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis.The Journal of cell biology · 1996Article
- Kinetochore microtubule dynamics and the metaphase-anaphase transition.The Journal of cell biology · 1995Article
- Intrinsic microtubule stability in interphase cells.The Journal of cell biology · 1994Article
- How the transition frequencies of microtubule dynamic instability (nucleation, catastrophe, and rescue) regulate microtubule dynamics in interphase and mitosis: analysis using a Monte Carlo computer simulation.Molecular biology of the cell · 1993Article
- Relationship between the Golgi complex and microtubules enriched in detyrosinated or acetylated alpha-tubulin: studies on cells recovering from nocodazole and cells in the terminal phase of cytokinesis.Cell and tissue research · 1993Article
- Do photobleached fluorescent microtubules move?: re-evaluation of fluorescence laser photobleaching both in vitro and in growing Xenopus axon.The Journal of cell biology · 1993Article
- Observation and quantification of individual microtubule behavior in vivo: microtubule dynamics are cell-type specific.The Journal of cell biology · 1993Article
- Microtubule dissassembly in vivo: intercalary destabilization and breakdown of microtubules in the heliozoan Actinocoryne contractilis.The Journal of cell biology · 1992Article
- Differential behavior of photoactivated microtubules in growing axons of mouse and frog neurons.The Journal of cell biology · 1992Article
- Mechanism of centrosome positioning during the wound response in BSC-1 cells.The Journal of cell biology · 1992Article
- The CDC20 gene product of Saccharomyces cerevisiae, a beta-transducin homolog, is required for a subset of microtubule-dependent cellular processes.Molecular and cellular biology · 1991Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Clarification of the mechanism of microtubule dynamics requires an analysis of the microtubule pattern at two time points in the same cell with single fiber resolution. Single microtubule resolution was obtained by microinjection of haptenized tubulin (fluorescein-tubulin) and subsequent indirect immunofluorescence with an antifluorescein antibody. The two time points in a single cell were, first, the time of photobleaching fluorescein-tubulin, and second, the time of fixation. The pattern of fluorescence replacement in the bleached zone during this time interval revealed the relevant mechanisms. In fibroblasts, microtubule domains in the bleached zone are replaced microtubule by microtubule and not by mechanisms that affect all microtubules simultaneously. Of the models we consider, treadmilling and subunit exchange along the length do not account for this observation, but dynamic instability can since it suggests that growing and shrinking microtubules coexist. In addition, we show that the half-time for microtubule replacement is shortest at the leading edge. Dynamic instability accounts for this observation if in general microtubules do not catastrophically disassemble from the plus end, but instead have a significant probability of undergoing a transition to the growing phase before they depolymerize completely. This type of instability we call tempered rather than catastrophic because, through limited disassembly followed by regrowth, it will preferentially replace polymer domains at the ends of microtubules, thus accounting for the observation that the half-time of microtubule domain replacement is shorter with proximity to the leading edge.
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