ArticleMethods in molecular biology (Clifton, N.J.)2022
Protocols for In Vivo Doubled Haploid (DH) Technology in Maize Breeding: From Haploid Inducer Development to Haploid Genome Doubling.
Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- A two-stage screening approach integrated with GWAS for rare phenotypes such as spontaneous haploid genome doubling in maize.Frontiers in plant science · 2026Article
- Multi-trait ridge regression BLUP withFrontiers in plant science · 2025Article
- Dissecting the genetic variation of haploid frailty in maize for enhanced doubled haploid breeding.Frontiers in plant science · 2025Article
- A novel in vivo genome editing doubled haploid system for Zea mays L.Nature plants · 2024Article
- Haploid identification in maize.Frontiers in plant science · 2024Review
- Self-supervised maize kernel classification and segmentation for embryo identification.Frontiers in plant science · 2023Article
- Development of doubled haploid inducer lines facilitates selection of superior haploid inducers in maize.Frontiers in plant science · 2023Article
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7 authors.
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Abstract
Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here. The major steps involved in in vivo DH technology are haploid induction, haploid selection/identification, and haploid genome doubling. Herein, we elaborate on the various steps of DH technology in maize breeding from haploid induction to haploid genome doubling to produce DH lines. Detailed protocols on the following topics are discussed: in vivo haploid inducer line development, haploid selection using seed and root color markers and automated seed sorting based on embryo oil content using QSorter, artificial genome doubling, and the identification of genotypes with spontaneous haploid genome doubling (SHGD) ability.
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