ArticleAntonie van Leeuwenhoek2022
Isolation of Hermetia illucens larvae core gut microbiota by two different cultivation strategies.
Article in Antonie van Leeuwenhoek, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- From Wild to Farm: Gut Bacteriome Differences and Probiotic Potential of Pantoea Agglomerans in Two-Spotted Cricket (Gryllus Bimaculatus) Rearing.Probiotics and antimicrobial proteins · 2026Article
- Selection andACS agricultural science & technology · 2026Article
- Multi-Condition Cultivation Reveals the Host Plant-Dependent Gut Bacteria Diversity in Tomato Leafminer (Insects · 2026Article
- Diet chemistry and cross-kingdom microbiota associate with black soldier fly larvae performance on regional side streams.Animal microbiome · 2026Article
- Control efficiency and Huanglongbing resistance-related clues mediated by novelFrontiers in microbiology · 2026Article
- Intestinal Microecological Mechanisms of Aflatoxin B1 Degradation by Black Soldier Fly Larvae (Animals : an open access journal from MDPI · 2025Review
- Investigating the Microbial Dynamics ofFoods (Basel, Switzerland) · 2025Article
- Unveiling Diversity and Function: Venom-Associated Microbes in Two Spiders, Heteropoda venatoria and Chilobrachys guangxiensis.Microbial ecology · 2024Article
- Black Soldier Fly (Animals : an open access journal from MDPI · 2024Article
- Genetics, age, and diet influence gut bacterial communities and performance of black soldier fly larvae (Hermetia illucens).Animal microbiome · 2024Article
- Evaluation of the Microbial Quality ofFoods (Basel, Switzerland) · 2024Article
- Effects of effective microorganisms on the physiological status, intestinal microbiome, and serum metabolites of Eriocheir sinensis.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Insect-based agri-food waste valorization: Agricultural applications and roles of insect gut microbiota.Environmental science and ecotechnology · 2024Review
- The Use of Probiotics during Rearing ofMicroorganisms · 2023Review
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Authors and funding
4 authors.
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Abstract
Hermetia illucens larvae (black soldier fly larvae, BSFL) convert efficiently organic waste to high quality biomass. To gain knowledge on the specific functions of gut microbes in this process it is a prerequisite to culture members of the core gut microbiota. Two different cultivation strategies were applied here for this purpose, a dilution-to-extinction cultivation and direct plating using six different media to culture aerobic heterotrophic bacteria. A total of 341 isolates were obtained by the dilution-to-extinction cultivation and 138 isolates by direct plating from guts of BSFL reared on chicken feed. Bacterial isolates were phylogenetically identified at the genus level by 16S rRNA gene sequencing (phylotyping) and differentiated at the strain level by genomic fingerprinting (genotyping). The main proportion of isolates was assigned to Proteobacteria, Firmicutes (Bacilli), and Actinobacteria. Predominant genera discussed in literature as member of a potential BSFL core gut microbiota, Providencia, Proteus, Morganella, Enterococcus, Bacillus, and members of the family Enterobacteriaceae, were isolated. A high intra-phylotype diversity was obtained by genomic fingerprinting which was especially enhanced by the dilution-to-extinction cultivation. This study showed that the application of different cultivation strategies including a dilution-to-extinction cultivation helps to culture a higher diversity of the BSFL gut microbiota and that genomic fingerprinting gives a better picture on the genetic diversity of cultured bacteria which cannot be covered by a 16S rRNA gene sequence based identification alone.
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