Evidence map›Paper›PMID 35459748›Full record

ArticleRNA (New York, N.Y.)2022

Metal content and kinetic properties of yeast RNA lariat debranching enzyme Dbr1.

Nathaniel E Clark, Adam Katolik, Allison J Taggart, Luke Buerer, Stephen P Holloway, Nathaniel Miller, John D Phillips, Colin P Farrell, Masad J Damha, William G Fairbrother

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

  1. Cryptic intronic transcriptional initiation generates efficient endogenous mRNA templates for C9orf72-associated RAN translation.Proceedings of the National Academy of Sciences of the United States of America · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 2 countries.

Nathaniel E ClarkDepartment of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02903, USA.
Adam KatolikDepartment of Chemistry, McGill University, Montreal, Quebec H3A 0B8, Canada.
Allison J TaggartDepartment of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02903, USA.
Luke BuererDepartment of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02903, USA.
Stephen P HollowayDepartment of Biochemistry and Structural Biology, University of Texas Health Science Center, San Antonio, Texas 78229, USA.
Nathaniel MillerDepartment of Geological Sciences, University of Texas Austin, Austin, Texas 78712, USA.
John D PhillipsDepartment of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
Colin P FarrellDepartment of Internal Medicine, University of Utah School of Medicine, Salt Lake City, Utah 84132, USA.
Masad J DamhaDepartment of Chemistry, McGill University, Montreal, Quebec H3A 0B8, Canada.
William G FairbrotherDepartment of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02903, USA.
Brown University · USMcGill University · CAUniversity of Utah · USRTX (United States) · USThe University of Texas at Austin · USThe University of Texas Health Science Center at San Antonio · US

Funding

Discovering Splicing Defects in Human GenesR01GM127472 · NIGMS · BROWN UNIVERSITY · PI William G Fairbrother · 2018 to 2026
$5.9M
A genomic approach to studying the life cycle of intron lariatsR01GM105681 · NIGMS · BROWN UNIVERSITY · PI FAIRBROTHER, WILLIAM G · 2014 to 2022
$3.7M
NIGMS NIH HHS R01 GM105681NIGMS NIH HHS R01 GM127472
6 · The paper itself

Abstract

In eukaryotic cells, intron lariats produced by the spliceosome contain a 2'5' phosphodiester linkage. The RNA lariat debranching enzyme, Dbr1, is the only enzyme known to hydrolyze this bond. Dbr1 is a member of the metallophosphoesterase (MPE) family of enzymes, and recent X-ray crystal structures and biochemistry data demonstrate that Dbr1 from

Indexed as

Escherichia coliSaccharomyces cerevisiaeHumansIntronsMetalsRNARNA NucleotidyltransferasesDbr1 protein, humanlariat debranching enzymeMetalsRNARNA Nucleotidyltransferasesdebranching enzymeintronslariatsmetalloenzymesmetallophosphoesteraseRNA

Identifiers

PMID35459748
PMCPMC9202583
OpenAlexW4224325804

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.