Evidence map›Paper›PMID 35458439›Full record

ArticleViruses2022

Filter Papers to Collect Blood Samples from Dogs: An Easier Way to Monitor the Mass Vaccination Campaigns against Rabies?

Marine Wasniewski, Jacques Barrat, Samia Ben Maiez, Habib Kharmachi, Mariem Handous, Florence Cliquet

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Marine WasniewskiANSES, Nancy Laboratory for Rabies and Wildlife, European Union Reference Laboratory for Rabies Serology, European Union Reference Laboratory for Rabies, WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, Technopôle Agricole et Vétérinaire, CS 40009 Malzeville, France.ORCID 0000-0001-5054-3634
Jacques BarratANSES, Nancy Laboratory for Rabies and Wildlife, European Union Reference Laboratory for Rabies Serology, European Union Reference Laboratory for Rabies, WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, Technopôle Agricole et Vétérinaire, CS 40009 Malzeville, France.
Samia Ben MaiezLaboratory of Rabies, Pasteur Institute of Tunis, 13 Place Pasteur, Tunis 1002, Tunisia.
Habib KharmachiLaboratory of Rabies, Pasteur Institute of Tunis, 13 Place Pasteur, Tunis 1002, Tunisia.
Mariem HandousLaboratory of Rabies, Pasteur Institute of Tunis, 13 Place Pasteur, Tunis 1002, Tunisia.ORCID 0000-0002-7494-0463
Florence CliquetANSES, Nancy Laboratory for Rabies and Wildlife, European Union Reference Laboratory for Rabies Serology, European Union Reference Laboratory for Rabies, WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, Technopôle Agricole et Vétérinaire, CS 40009 Malzeville, France.
Agence Nationale de Sécurité Sanitaire de l’Alimentation, de l’Environnement et du Travail · FRInstitut Pasteur de Tunis · TN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rabies is a deadly viral disease present mainly in low-income countries of Africa and Asia. Dogs are the main reservoir and the source of human deaths. Mass vaccination campaigns of dogs are pivotal to achieve rabies elimination. The monitoring of the immune response of the dog population is necessary to evaluate the effectiveness of these campaigns, taking into account field conditions. This study explores the feasibility and the performance of a new tool using filter papers (FPs) to collect blood samples associated with an Enzyme-Linked ImmunoSorbent Assay (ELISA) titration of rabies antibodies in dogs. A total of 216 eluates from FP samples were collected from 111 dogs kept in experimental facilities in France and 29 dogs from the field in Tunisia. Sera were also analyzed using both the Fluorescence Antibody Virus Neutralization test (FAVNt) and ELISA. A high specificity (98.0%) was obtained by testing FP blood eluates from 51 unvaccinated dogs, with the results compared with those of FAVNt and ELISA on serum samples. The coefficients of concordance between FP eluates and tested sera were 88.9% for FAVNt and 88.0% for ELISA. Blood filter papers coupled with the titration of rabies antibodies by ELISA provide a reliable, simple, and effective solution to overcome the issues of the logistics and transport of samples, especially in low-income countries.

Indexed as

Dog DiseasesRabiesRabies VaccinesRabies virusAnimalsAntibodies, ViralDogsEnzyme-Linked Immunosorbent AssayImmunization ProgramsSensitivity and SpecificityTunisiaVaccinationAntibodies, ViralRabies VaccinesdogsELISAFAVN testfilter papermass vaccinationrabies virus

Identifiers

PMID35458439
PMCPMC9029112
OpenAlexW4220988986

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.