ArticleInternational journal of molecular sciences2022
Quality Control of Bacterial Extracellular Vesicles with Total Protein Content Assay, Nanoparticles Tracking Analysis, and Capillary Electrophoresis.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Bacterial Extracellular Vesicles at the Crossroads of Immune Regulation and Biofilm Dynamics: Biogenesis, Comparative Analysis, and Translational Challenges.Biomolecules · 2026Review
- Gut microbiota-derived extracellular vesicles: bridging microbial-host crosstalk in metabolic disorders.Cell communication and signaling : CCS · 2026Review
- Fluconazole tolerance is associated with altered extracellular vesicle-mediated epithelial immune responses inFrontiers in microbiology · 2026Article
- Enhanced Separation of Extracellular Vesicles Using Capillary Isotachophoresis With Spacer Compounds.Electrophoresis · 2025Article
- Exosome-Based Therapeutics in Dermatology.Biomaterials research · 2025Review
- Characterization of extracellular vesicles by capillary zone electrophoresis: A novel concept for characterization of a next-generation drug delivery platform.Journal of pharmaceutical analysis · 2024Review
- Loss of Lipooligosaccharide Synthesis inInternational journal of molecular sciences · 2024Article
- Membrane Vesicles of Clostridioides difficile and Other Clostridial Species.Advances in experimental medicine and biology · 2024Article
- Role of probiotic extracellular vesicles in inter-kingdom communication and current technical limitations in advancing their therapeutic utility.Extracellular vesicles and circulating nucleic acids · 2024Review
- Protein overproduction alters exosome secretion in Chinese hamster ovary cells.Analytical and bioanalytical chemistry · 2023Article
- Extracellular Vesicles in Microbes, Pathogens, and Infectious Diseases.International journal of molecular sciences · 2023Article
- Deciphering psychobiotics' mechanism of action: bacterial extracellular vesicles in the spotlight.Frontiers in microbiology · 2023Review
- The role of extracellular vesicles in periodontitis: pathogenesis, diagnosis, and therapy.Frontiers in immunology · 2023Review
- Assessment of Small Cellular Particles from Four Different Natural Sources and Liposomes by Interferometric Light Microscopy.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Extracellular vesicles (EVs) were isolated from Pectobacterium zantedeschiae culturing media using direct ultracentrifugation (UC), iodixanol cushion ultracentrifugation (ICUC), and iodixanol density gradient ultracentrifugation (IDGUC) techniques. The isolates were characterized with total protein content assay (bicinchoninic acid assay, BCA), nanoparticles tracking analysis (NTA), and capillary electrophoresis (CE). A satisfactory correlation (R2 > 0.94) between quantitative results obtained with BCA, NTA and CE was achieved only for isolates obtained with the IDGUC. The correlation between protein content and CE was proved to be related to the isolates’ purity. The NTA was found unable to provide reliable information on EVs quantity in samples isolated with UC and ICUC, due to the co-isolated particulate impurities. Moreover, the work reports polysaccharides, used as culturing media components, as a potential source of bias of quantitation with total protein content assay and NTA. The study demonstrates the advantageous selectivity of CE in quality control of EVs and its ability to differentiate subpopulations of EVs of Pectobacterium.
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Registered trials
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