Evidence map›Paper›PMID 35456956›Full record

ArticleInternational journal of molecular sciences2022

Methylglyoxal Impairs Sister Chromatid Separation in Lymphocytes.

Leigh Donnellan, Clifford Young, Bradley S Simpson, Varinderpal S Dhillon, Maurizio Costabile, Peter Hoffmann, Michael Fenech, Permal Deo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Leigh DonnellanHealth and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Clifford YoungClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Bradley S SimpsonHealth and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0002-6356-962X
Varinderpal S DhillonHealth and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Maurizio CostabileHealth and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0002-2162-6497
Peter HoffmannClinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Michael FenechHealth and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.
Permal DeoHealth and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide 5000, Australia.ORCID 0000-0002-6477-9127
University of South Australia · AUSouth Australia Pathology · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The accurate segregation of sister chromatids is complex, and errors that arise throughout this process can drive chromosomal instability and tumorigenesis. We recently showed that methylglyoxal (MGO), a glycolytic by-product, can cause chromosome missegregation events in lymphocytes. However, the underlying mechanisms of this were not explored. Therefore, in this study, we utilised shotgun proteomics to identify MGO-modified proteins, and label-free quantitation to measure changes in protein abundance following exposure to MGO. We identified numerous mitotic proteins that were modified by MGO, including those involved in the separation and cohesion of sister chromatids. Furthermore, the protein abundance of Securin, an inhibitor of sister chromatid separation, was increased following treatment with MGO. Cytological examination of chromosome spreads showed MGO prevented sister chromatid separation, which was associated with the formation of complex nuclear anomalies. Therefore, results from this study suggest MGO may drive chromosomal instability by preventing sister chromatid separation.

Indexed as

ChromatidsPyruvaldehydeCell Cycle ProteinsChromosomal InstabilityChromosome SegregationHumansLymphocytesMagnesium OxideCell Cycle ProteinsMagnesium OxidePyruvaldehydechromosomal instabilitymethylglyoxalproteomicssister chromatid separation

Identifiers

PMID35456956
PMCPMC9030103
OpenAlexW4224306501

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.