ArticleInternational journal of molecular sciences2022
Carbon Nanotube-Mediated Plasmid DNA Delivery in Rice Leaves and Seeds.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 57 citations in OpenAlex.
- Overexpression ofGenes · 2026Article
- A Plant-Derived Arabinoxylan Platform for Biomolecule Delivery into Plant Cells.Journal of agricultural and food chemistry · 2026Article
- Seeds and nanomaterials: seed-assisted synthesis, nanotoxicity, and seed-associated nanotechnology innovations.Journal of experimental botany · 2026Review
- A new use of Agrobacterium plant growth regulator genes for plant bioengineering.Frontiers in plant science · 2026Article
- Single-walled carbon nanotube-mediated plasmid DNA delivery in the model monocotTurkish journal of biology = Turk biyoloji dergisi · 2026Article
- High aspect ratio graphene oxide: a highly efficient plasmid DNA deliverer for plant seed.Frontiers in plant science · 2026Article
- Article
- Agricultural nanotechnology for a safe and sustainable future: current status, challenges, and beyond.Journal of the science of food and agriculture · 2025Review
- Pigments to precision: RUBY aiding genetic transformation and genome editing in wheat and barley.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- DNA delivery into plant tissues using carbon dots made from citric acid and β-alanine.Frontiers in chemistry · 2025Article
- Carbon Nanomaterial Fluorescent Probes and Their Biological Applications.Chemical reviews · 2024Review
- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- Review
- Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells.International journal of molecular sciences · 2023Review
- Guidelines for Performing CRISPR/Cas9 Genome Editing for Gene Validation and Trait Improvement in Crops.Plants (Basel, Switzerland) · 2023Review
- Technological Development and Application of Plant Genetic Transformation.International journal of molecular sciences · 2023Review
- CRISPR-Based Genome Editing Tools: An Accelerator in Crop Breeding for a Changing Future.International journal of molecular sciences · 2023Review
- Recent advances in the delivery and applications of nonviral CRISPR/Cas9 gene editing.Drug delivery and translational research · 2023Review
- Functional Allele Validation by Gene Editing to Leverage the Wealth of Genetic Resources for Crop Improvement.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
CRISPR-Cas gene editing technologies offer the potential to modify crops precisely; however, in vitro plant transformation and regeneration techniques present a bottleneck due to the lengthy and genotype-specific tissue culture process. Ideally, in planta transformation can bypass tissue culture and directly lead to transformed plants, but efficient in planta delivery and transformation remains a challenge. This study investigates transformation methods that have the potential to directly alter germline cells, eliminating the challenge of in vitro plant regeneration. Recent studies have demonstrated that carbon nanotubes (CNTs) loaded with plasmid DNA can diffuse through plant cell walls, facilitating transient expression of foreign genetic elements in plant tissues. To test if this approach is a viable technique for in planta transformation, CNT-mediated plasmid DNA delivery into rice tissues was performed using leaf and excised-embryo infiltration with reporter genes. Quantitative and qualitative data indicate that CNTs facilitate plasmid DNA delivery in rice leaf and embryo tissues, resulting in transient GFP, YFP, and GUS expression. Experiments were also initiated with CRISPR-Cas vectors targeting the
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.