ArticlePathogens (Basel, Switzerland)2022
Development of a Real-Time Recombinase Polymerase Amplification Assay for the Rapid Detection of African Swine Fever Virus Genotype I and II.
Article in Pathogens (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Evaluation of the Flashtest multiplex qPCR system for rapid pre-laboratory screening for African swine fever infection.Journal of veterinary research · 2026Article
- Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-G.PloS one · 2025Article
- Strategic nucleic acid detection approaches for diagnosing African swine fever (ASF): navigating disease dynamics.Veterinary research · 2024Review
- Validation of a direct multiplex real-time reverse transcription PCR assay for rapid detection of African swine fever virus using swine field samples in Vietnam.BMC research notes · 2024Article
- Development of a novel sensitive single-tube nested PCR assay for the detection of African swine fever virus.Archives of virology · 2024Article
- On-site detection and differentiation of African swine fever virus variants using an orthogonal CRISPR-Cas12b/Cas13a-based assay.iScience · 2024Article
- A triplex crystal digital PCR for the detection of genotypes I and II African swine fever virus.Frontiers in veterinary science · 2024Article
- Advanced Strategies for Developing Vaccines and Diagnostic Tools for African Swine Fever.Viruses · 2023Review
- African Swine Fever: Epidemiology, the Design of New Diagnostic Methods, and Vaccine Development.Pathogens (Basel, Switzerland) · 2023Article
- Novel sensitive isothermal-based diagnostic technique for the detection of African swine fever virus.Archives of virology · 2023Article
- Development of a TaqMan-Probe-Based Multiplex Real-Time PCR for the Simultaneous Detection of African Swine Fever Virus, Porcine Circovirus 2, and Pseudorabies Virus in East China from 2020 to 2022.Veterinary sciences · 2023Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
African swine fever (ASF) is a contagious viral disease in pigs and wild boars which poses a major threat to the pig industry. Rapid and accurate diagnosis is necessary to control ASF. Hence, we developed a rapid diagnostic method using a recombinase polymerase amplification (RPA) assay targeting the conserved sequences of CP204L (p30) thatcan rapidly detect ASF virus (ASFV) genotype strains I and II. The lower detection limit of the real-time RPA assay was 5 × 101 copies per reaction. The real-time RPA assay effectively detected ASFV isolates and clinical specimens belonging to ASFV genotypes I and II. The sensitivity and specificity of the assay were 96.8% (95% confidence interval (CI): 83.3−99.9) and 100% (95% CI: 88.4−100.0), respectively. The agreement between the real-time RPA assay and a reference commercial real-time quantitative polymerase chain reaction (qPCR) was 100%. The real-time RPA assay had a detection time of 6.0 min (95% CI: 5.7−6.2), which was significantly shorter than that of qPCR (49 min; 95% CI: 47.4−50.6; p < 0.001). Thus, the developed real-time RPA assay is a rapid and accurate diagnostic tool for detecting ASFV genotypes I and II.
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