Evidence map›Paper›PMID 35453719›Full record

ArticleBiology2022

Genome Characterisation of an Isoprene-Degrading

Toungporn Uttarotai, Sawannee Sutheeworapong, Andrew T Crombie, J Colin Murrell, Wuttichai Mhuantong, Nuttapol Noirungsee, Sunanta Wangkarn, Sakunnee Bovonsombut, Terry J McGenity, Thararat Chitov

Open access · goldAbstract read
In one paragraph

Article in Biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.8field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Biologically active metabolites ofFuture science OA · 2024
    Review
  6. Article
  7. Analysis of Essential Isoprene Metabolic Pathway Proteins inApplied and environmental microbiology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 2 countries.

Toungporn UttarotaiDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Sawannee SutheeworapongSchool of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
Andrew T CrombieSchool of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
J Colin MurrellSchool of Environmental Sciences, University of East Anglia, Norwich NR4 7TJ, UK.
Wuttichai MhuantongEnzyme Technology Research Team, Biorefinery and Bioproduct Technology Research Group, National Center for Genetic Engineering and Biotechnology, Pathumthani 12120, Thailand.ORCID 0000-0002-3355-0071
Nuttapol NoirungseeDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Sunanta WangkarnDepartment of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Sakunnee BovonsombutDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Terry J McGenitySchool of Life Sciences, University of Essex, Colchester CO4 3SQ, UK.ORCID 0000-0002-1497-8822
Thararat ChitovDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Chiang Mai University · THUniversity of East Anglia · GBUniversity of Essex · GBKing Mongkut's University of Technology Thonburi · THNational Center for Genetic Engineering and Biotechnology · TH

Funding

Biodiversity-based Economy Development Office (Public Organisation) (BEDO) 36/2562European Research Council IsoMet 694578Natural Environment Research Council NE/J009555/1
6 · The paper itself

Abstract

Isoprene is a climate-active biogenic volatile organic compound (BVOC), emitted into the atmosphere in abundance, mainly from terrestrial plants. Soil is an important sink for isoprene due to its consumption by microbes. In this study, we report the ability of a soil bacterium to degrade isoprene. Strain 13f was isolated from soil beneath wild Himalayan cherry trees in a tropical restored forest. Based on phylogenomic analysis and an Average Nucleotide Identity score of >95%, it most probably belongs to the species Alcaligenes faecalis. Isoprene degradation by Alcaligenes sp. strain 13f was measured by using gas chromatography. When isoprene was supplied as the sole carbon and energy source at the concentration of 7.2 × 105 ppbv and 7.2 × 106 ppbv, 32.6% and 19.6% of isoprene was consumed after 18 days, respectively. Genome analysis of Alcaligenes sp. strain 13f revealed that the genes that are typically found as part of the isoprene monooxygenase gene cluster in other isoprene-degrading bacteria were absent. This discovery suggests that there may be alternative pathways for isoprene metabolism.

Indexed as

Alcaligenesclimate-active gasgenomeisopreneisoprene degradation

Identifiers

PMID35453719
PMCPMC9030188
OpenAlexW4220977824

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.