Evidence map›Paper›PMID 35452675›Full record

ArticleThe Journal of biological chemistry2022

Development of antiviral carbon quantum dots that target the Japanese encephalitis virus envelope protein.

Han-Hsiang Chen, Chin-Jung Lin, Anisha Anand, Han-Jia Lin, Hung-Yun Lin, Ju-Yi Mao, Pei-Hua Wang, Yufeng Jane Tseng, Wen-Shyong Tzou, Chih-Ching Huang and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
9.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 53 citations in OpenAlex.

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  20. Carbon dots nanozyme for anti-inflammatory therapyFrontiers in bioengineering and biotechnology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Han-Hsiang ChenDivision of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
Chin-Jung LinDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
Anisha AnandDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
Han-Jia LinDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan.
Hung-Yun LinDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
Ju-Yi MaoDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
Pei-Hua WangGraduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan.
Yufeng Jane TsengGraduate Institute of Biomedical Electronics and Bioinformatics, National Taiwan University, Taipei, Taiwan; Department of Computer Science and Information Engineering, National Taiwan University, Taipei, Taiwan.
Wen-Shyong TzouDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan.
Chih-Ching HuangDepartment of Bioscience and Biotechnology, National Taiwan Ocean University, Keelung, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan. Electronic address: huanging@ntou.edu.tw.
Robert Y L WangDivision of Microbiology and Immunology, Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Department of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, Taiwan; Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial and Children's Hospital, Linkou, Taiwan; Kidney Research Center and Department of Nephrology, Chang Gung Memorial Hospital, Linkou, Taiwan. Electronic address: yuwang@mail.cgu.edu.tw.
National Taiwan Ocean University · TWChang Gung University · TWNational Taiwan University · TWKaohsiung Medical University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Japanese encephalitis is a mosquito-borne disease caused by the Japanese encephalitis virus (JEV) that is prevalent in Asia and the Western Pacific. Currently, there is no effective treatment for Japanese encephalitis. Curcumin (Cur) is a compound extracted from the roots of Curcuma longa, and many studies have reported its antiviral and anti-inflammatory activities. However, the high cytotoxicity and very low solubility of Cur limit its biomedical applications. In this study, Cur carbon quantum dots (Cur-CQDs) were synthesized by mild pyrolysis-induced polymerization and carbonization, leading to higher water solubility and lower cytotoxicity, as well as superior antiviral activity against JEV infection. We found that Cur-CQDs effectively bound to the E protein of JEV, preventing viral entry into the host cells. In addition, after continued treatment of JEV with Cur-CQDs, a mutant strain of JEV was evolved that did not support binding of Cur-CQDs to the JEV envelope. Using transmission electron microscopy, biolayer interferometry, and molecular docking analysis, we revealed that the S123R and K312R mutations in the E protein play a key role in binding Cur-CQDs. The S123 and K312 residues are located in structural domains II and III of the E protein, respectively, and are responsible for binding to receptors on and fusing with the cell membrane. Taken together, our results suggest that the E protein of flaviviruses represents a potential target for the development of CQD-based inhibitors to prevent or treat viral infections.

Indexed as

Encephalitis, JapaneseEncephalitis Virus, JapaneseQuantum DotsAnimalsAntiviral AgentsCarbonMolecular Docking SimulationViral Envelope ProteinsAntiviral AgentsCarbonViral Envelope Proteinscarbon quantum dotscurcuminJapanese encephalitis virusviral entryviral envelope protein

Identifiers

PMID35452675
PMCPMC9123278
OpenAlexW4224280962

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.