Evidence map›Paper›PMID 35452597›Full record

ArticleStem cell reports2022

Argonaute proteins regulate a specific network of genes through KLF4 in mouse embryonic stem cells.

Madlen Müller, Moritz Schaefer, Tara Fäh, Daniel Spies, Victoria Hermes, Richard Patryk Ngondo, Rodrigo Peña-Hernández, Raffaella Santoro, Constance Ciaudo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Madlen MüllerSwiss Federal Institute of Technology Zurich, Institute of Molecular Health Sciences, Chair of RNAi and Genome Integrity, Zurich, Switzerland; Life Science Zurich Graduate School, University of Zurich, Zurich, Switzerland.
Moritz SchaeferSwiss Federal Institute of Technology Zurich, Institute of Molecular Health Sciences, Chair of RNAi and Genome Integrity, Zurich, Switzerland; Life Science Zurich Graduate School, University of Zurich, Zurich, Switzerland.
Tara FähSwiss Federal Institute of Technology Zurich, Institute of Molecular Health Sciences, Chair of RNAi and Genome Integrity, Zurich, Switzerland.
Daniel SpiesSwiss Federal Institute of Technology Zurich, Institute of Molecular Health Sciences, Chair of RNAi and Genome Integrity, Zurich, Switzerland.
Victoria HermesSwiss Federal Institute of Technology Zurich, Institute of Molecular Health Sciences, Chair of RNAi and Genome Integrity, Zurich, Switzerland.
Richard Patryk NgondoSwiss Federal Institute of Technology Zurich, Institute of Molecular Health Sciences, Chair of RNAi and Genome Integrity, Zurich, Switzerland.
Rodrigo Peña-HernándezDepartment of Molecular Mechanisms of Disease, DMMD, University of Zurich, Zurich, Switzerland.
Raffaella SantoroDepartment of Molecular Mechanisms of Disease, DMMD, University of Zurich, Zurich, Switzerland.
Constance CiaudoSwiss Federal Institute of Technology Zurich, Institute of Molecular Health Sciences, Chair of RNAi and Genome Integrity, Zurich, Switzerland. Electronic address: cciaudo@ethz.ch.
École Polytechnique Fédérale de Lausanne · CHUniversity of Zurich · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Argonaute proteins (AGOs) are well known for their role in post-transcriptional gene silencing in the microRNA (miRNA) pathway. Here we show that in mouse embryonic stem cells, AGO1&2 serve additional functions that go beyond the miRNA pathway. Through the combined deletion of both Agos, we identified a specific set of genes that are uniquely regulated by AGOs but not by the other miRNA biogenesis factors. Deletion of Ago2&1 caused a global reduction of the repressive histone mark H3K27me3 due to downregulation at protein levels of Polycomb repressive complex 2 components. By integrating chromatin accessibility, prediction of transcription factor binding sites, and chromatin immunoprecipitation sequencing data, we identified the pluripotency factor KLF4 as a key modulator of AGO1&2-regulated genes. Our findings revealed a novel axis of gene regulation that is mediated by noncanonical functions of AGO proteins that affect chromatin states and gene expression using mechanisms outside the miRNA pathway.

Indexed as

Argonaute ProteinsMicroRNAsAnimalsChromatinKruppel-Like Factor 4MiceMouse Embryonic Stem CellsPolycomb Repressive Complex 2Argonaute ProteinsChromatinKlf4 protein, mouseKruppel-Like Factor 4MicroRNAsPolycomb Repressive Complex 2ArgonautesCTCFH3K27me3integrative analysisKLF4PRC2

Identifiers

PMID35452597
PMCPMC9133645
OpenAlexW4225796322

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.