ArticleProceedings of the National Academy of Sciences of the United States of America2022
Protein and lipid mass concentration measurement in tissues by stimulated Raman scattering microscopy.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers, 1 of them a synthesis that pooled it.
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Who cites it
71 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- Macromolecular Crowding in Cytoplasm and Cellular Organelles.Sub-cellular biochemistry · 2025Pooled it
- Longitudinal live-cell chemical imaging using coherent Raman scattering microscopy.The Analyst · 2026Review
- Role of GPCR41 silencing in conjugated linoleic acid-induced adipogenesis of bovine intramuscular preadipocytes.In vitro cellular & developmental biology. Animal · 2026Article
- PLANCK: super-multiplex optical imaging without labeling.bioRxiv : the preprint server for biology · 2026Article
- Getting nuclear size just right - emerging mechanisms regulating nuclear scaling and morphology.Journal of cell science · 2026Review
- Single-Cell Metabolic Imaging and Digital Scoring of Fat Tissue Remodeling by Label-Free Metabolic Microscopy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Kidney tissue characterization using normalized raman imaging and segment-anything.Scientific reports · 2026Article
- Inertial sensing of water content in tumor spheroids.Science advances · 2026Article
- Single-cell structural lipidomics using a miniature dual-LIT mass spectrometer.Chemical science · 2026Article
- How does cytoplasmic crowding affect reaction rates?Molecular cell · 2026Review
- Article
- Stimulated Raman Scattering Imaging Enabled Biomarker Discovery for Precision Medicine.Chemical & biomedical imaging · 2025Review
- Stimulated Raman Scattering (SRS) Microscopy Enables Visualization and Quantification of Cryoprotective Agent Concentration inAnalytical chemistry · 2025Article
- Tumour Cell Size Control and Its Impact on Tumour Cell Function.Cell proliferation · 2025Review
- Raman-based label-free microscopic analysis of the pancreas in living zebrafish larvae.FEBS open bio · 2025Article
- The Wnt/APC destruction complex targets SREBP2 in a β-catenin-independent pathway to control cholesterol metabolism.bioRxiv : the preprint server for biology · 2025Article
- Artificial Intelligence-Powered Raman Spectroscopy through Open Science and FAIR Principles.ACS nano · 2025Review
- High-throughput single-cell density measurements enable dynamic profiling of immune cell and drug response from patient samples.Nature biomedical engineering · 2025Article
- Normalized Raman Imaging for Studies of Tissue Physiology of the Kidney.bioRxiv : the preprint server for biology · 2025Article
- Imaging Cellular Metabolic Rewiring with SuMMIT-SRS.bioRxiv : the preprint server for biology · 2025Article
11 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Cell mass and chemical composition are important aggregate cellular properties that are especially relevant to physiological processes, such as growth control and tissue homeostasis. Despite their importance, it has been difficult to measure these features quantitatively at the individual cell level in intact tissue. Here, we introduce normalized Raman imaging (NoRI), a stimulated Raman scattering (SRS) microscopy method that provides the local concentrations of protein, lipid, and water from live or fixed tissue samples with high spatial resolution. Using NoRI, we demonstrate that protein, lipid, and water concentrations at the single cell are maintained in a tight range in cells under the same physiological conditions and are altered in different physiological states, such as cell cycle stages, attachment to substrates of different stiffness, or by entering senescence. In animal tissues, protein and lipid concentration varies with cell types, yet an unexpected cell-to-cell heterogeneity was found in cerebellar Purkinje cells. The protein and lipid concentration profile provides means to quantitatively compare disease-related pathology, as demonstrated using models of Alzheimer’s disease. This demonstration shows that NoRI is a broadly applicable technique for probing the biological regulation of protein mass, lipid mass, and water mass for studies of cellular and tissue growth, homeostasis, and disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.