ArticleJournal of clinical periodontology2022
Discovery, validation, and diagnostic ability of multiple protein-based biomarkers in saliva and gingival crevicular fluid to distinguish between health and periodontal diseases.
Article in Journal of clinical periodontology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 3 syntheses or guidelines pooled it, 68 citations in OpenAlex.
- MS-Based Crevicular Fluid Proteomics for the Study of Periodontal and Peri-Implant Conditions: A Systematic Review.Journal of proteome research · 2026Pooled it
- Salivary Proteomics for Detecting Novel Biomarkers of Periodontitis: A Systematic Review.Journal of periodontal research · 2025Pooled it
- Mass Spectrometry-Based Proteomics for Discovering Salivary Biomarkers in Periodontitis: A Systematic Review.International journal of molecular sciences · 2023Pooled it
- Salivary asprosin, IL-39, IL-40, and IL-1β levels in diabetic patients with periodontitis: A cross-sectional analysis.Clinical oral investigations · 2026Trial
- Diagnostic and Therapeutic Approaches in Periodontology: From Traditional Concepts to Modern Innovations.Biomedicines · 2026Review
- Potential of Saliva in Stroke Patients: A Review.International journal of molecular sciences · 2026Review
- aMMP-8 self-testing and self-administered questionnaires for periodontitis screening: A diagnostic trial in a Chinese population.Journal of periodontology · 2026Article
- Combination of Lacticaseibacillus rhamnosus and Weissella confusa treatment improves ligature-induced periodontitis in rats via immune modulation and inhibition of oral pathogens.BMC oral health · 2026Article
- Salivary Proteomic Signatures in Pregnant Women With Excessive Gingival Bleeding.Oral diseases · 2026Observational
- Advanced Diagnostic Technologies and Molecular Biomarkers in Periodontitis: Systemic Health Implications and Translational Perspectives.Journal of clinical medicine · 2026Review
- Association of serum 25(OH)D with Cathepsin K levels in gingival crevicular fluid and saliva in periodontal health and disease: a cross-sectional study.BMC oral health · 2026Article
- Fibrinogen was associated with subgingival microbiome in periodontal diseases: a pilot study.Journal of oral microbiology · 2026Article
- Global Salivary Proteomic Profiling of Individuals with the Co-Occurrence of Type 2 Diabetes Mellitus, Dyslipidemia, and Periodontitis.International journal of molecular sciences · 2025Article
- Establishing cut-off values for salivary MMP-8 and IL-1β in the diagnosis of active periodontal disease: a preliminary cohort study toward the development of a diagnostic kit.Journal of periodontal & implant science · 2025Article
- Salivary Biomarker Profile in Periodontal Diseases: A Cross-Sectional Study on Leptin, Adiponectin, and Calprotectin.Diagnostics (Basel, Switzerland) · 2025Article
- Saliva Versus All-Site Microbiome and Proteome Mapping in Periodontitis.Journal of clinical periodontology · 2025Article
- The Association of Periodontal Inflammation and Systemic Health Indicators: A Machine Learning Approach.Journal of clinical periodontology · 2025Article
- Recent Advances and Perspectives of Metabolomics-Based Investigations in Coronary Heart Disease.Current atherosclerosis reports · 2025Review
- Efficacy of Oral Intake of Hydrogen-Rich Jelly Intake on Gingival Inflammation: A Double-Blind, Placebo-Controlled and Exploratory Randomized Clinical Trial.Healthcare (Basel, Switzerland) · 2025Article
- Diagnostic Accuracy of Novel Protein Biomarkers in Saliva to Detect Periodontitis Using Untargeted 'SWATH' Mass Spectrometry.Journal of clinical periodontology · 2025Article
Corrections and comments
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Authors and funding
17 authors at 5 institutions in 2 countries.
Funding
Abstract
aimTo discover and validate differential protein biomarker expression in saliva and gingival crevicular fluid (GCF) to discriminate objectively between periodontal health and plaque-induced periodontal disease states. MATERIALS AND
methodsOne-hundred and ninety participants were recruited from two centres (Birmingham and Newcastle upon Tyne, UK) comprising healthy, gingivitis, periodontitis, and edentulous donors. Samples from the Birmingham cohort were analysed by quantitative mass spectrometry proteomics for biomarker discovery. Shortlisted candidate proteins were then verified by enzyme-linked immunosorbent assay in both cohorts. Leave-one-out cross validation logistic regression analysis was used to identify the best performing biomarker panels.
resultsNinety-five proteins were identified in both GCF and saliva samples, and 15 candidate proteins were selected based upon differences discovered between the donor groups. The best performing panels to distinguish between: health or gingivitis and periodontitis contained matrix metalloproteinase-9 (MMP9), S100A8, alpha-1-acid glycoprotein (A1AGP), pyruvate kinase, and age (area under the curve [AUC] 0.970); health and gingivitis contained MMP9, S100A8, A1AGP, and pyruvate kinase, but not age (AUC 0.768); and mild to moderate and advanced periodontitis contained MMP9, S100A8, A1AGP, pyruvate kinase, and age (AUC 0.789).
conclusionsBiomarker panels containing four proteins with and without age as a further parameter can distinguish between periodontal health and disease states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.