ArticleBiophysical journal2022
Measurement of the persistence length of cytoskeletal filaments using curvature distributions.
Article in Biophysical journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 21 citations in OpenAlex.
- CaMKII generates actin bundle morphology and mechanics distinct from canonical bivalent cross-linkers.bioRxiv : the preprint server for biology · 2026Article
- Spatial confinement shapes organelle architecture and remodeling in axons.bioRxiv : the preprint server for biology · 2026Article
- Evolutionarily conserved short linear motifs drive actin filament binding.Nature cell biology · 2026Article
- Polyacid Solutions as an Analogue of a Neural Network.Polymers · 2026Article
- Development of a Microfluidics-Based Approach for Investigating Microtubule Polymer Mechanics.Journal of visualized experiments : JoVE · 2025Article
- Vimentin undergoes liquid-liquid phase separation to form droplets which wet and stabilize actin fibers.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Hydrolysis-deficient mosaic microtubules as faithful mimics of the GTP cap.Nature communications · 2025Article
- Cellular signalling protrusions enable dynamic distant contacts in spinal cord neurogenesis.Biology open · 2025Article
- Cellular mechanisms of traumatic brain injury.npj biological physics and mechanics · 2025Review
- Microtubule flexibility, microtubule-based nucleation and ROP pattern co-alignment enhance protoxylem microtubule patterning.Quantitative plant biology · 2025Article
- Stabilization of F-actin by Salmonella effector SipA resembles the structural effects of inorganic phosphate and phalloidin.Structure (London, England : 1993) · 2024Article
- Stabilization of F-actin bybioRxiv : the preprint server for biology · 2023Article
- Long-term cargo tracking reveals intricate trafficking through active cytoskeletal networks in the crowded cellular environment.Nature communications · 2023Article
- Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels.Nature communications · 2023Article
- Chiral and nematic phases of flexible active filaments.Nature physics · 2023Article
- Controlling the Rigidity of Kinesin-Propelled Microtubules in anACS omega · 2022Article
- Structure and assembly of double-headed Sendai virus nucleocapsids.Communications biology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Cytoskeletal filaments, such as microtubules and actin filaments, play important roles in the mechanical integrity of cells and the ability of cells to respond to their environment. Measuring the mechanical properties of cytoskeletal structures is crucial for gaining insight into intracellular mechanical stresses and their role in regulating cellular processes. One of the ways to characterize these mechanical properties is by measuring their persistence length, the average length over which filaments stay straight. There are several approaches in the literature for measuring filament deformations, such as Fourier analysis of images obtained using fluorescence microscopy. Here, we show how curvature distributions can be used as an alternative tool to quantify biofilament deformations, and investigate how the apparent stiffness of filaments depends on the resolution and noise of the imaging system. We present analytical calculations of the scaling curvature distributions as a function of filament discretization, and test our predictions by comparing Monte Carlo simulations with results from existing techniques. We also apply our approach to microtubules and actin filaments obtained from in vitro gliding assay experiments with high densities of nonfunctional motors, and calculate the persistence length of these filaments. The presented curvature analysis is significantly more accurate compared with existing approaches for small data sets, and can be readily applied to both in vitro and in vivo filament data through the use of the open-source codes we provide.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.