Evidence map›Paper›PMID 35449380›Full record

ArticleGeroScience2022

Epigenetic clock and methylation studies in marsupials: opossums, Tasmanian devils, kangaroos, and wallabies.

Steve Horvath, Amin Haghani, Joseph A Zoller, Ken Raj, Ishani Sinha, Todd R Robeck, Pete Black, Aidan Couzens, Clive Lau, Meghety Manoyan and 5 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in GeroScience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 18 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. The androgen clock is an epigenetic predictor of long-term male hormone exposure.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  8. Article
  9. Review
  10. Review
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 4 countries.

Steve HorvathHuman Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA. shorvath@mednet.ucla.edu.ORCID 0000-0002-4110-3589
Amin HaghaniHuman Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
Joseph A ZollerDepartment of Biostatistics, Fielding School of Public Health, University of California, Los Angeles, Los Angeles, CA, USA.
Ken RajRadiation Effects Department, Centre for Radiation, Chemical and Environmental Hazards, Public Health England, Chilton, Didcot, UK.
Ishani SinhaDepartment of Ecology and Evolutionary Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA.
Todd R RobeckZoological Operations, SeaWorld Parks and Entertainment, 8008 SeaWorld Drive, Orlando, FL, USA.
Pete BlackBusch Gardens Tampa, Tampa, FL, USA.
Aidan CouzensDepartment of Ecology and Evolutionary Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA.
Clive LauDepartment of Ecology and Evolutionary Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA.
Meghety ManoyanDepartment of Ecology and Evolutionary Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA.
Yadiamaris Aviles RuizCiencias Naturales, Universidad Interamericana de Puerto Rico, Bayamón, 00958, Puerto Rico.
Annais TalbottCiencias Naturales, Universidad Interamericana de Puerto Rico, Bayamón, 00958, Puerto Rico.
Katherine BelovSchool of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, Australia.
Carolyn J HoggSchool of Life and Environmental Sciences, The University of Sydney, Sydney, NSW, Australia. carolyn.hogg@sydney.edu.au.
Karen E SearsDepartment of Ecology and Evolutionary Biology, University of California at Los Angeles, Los Angeles, CA, 90095, USA. ksears@ucla.edu.
University of California, Los Angeles · USInterAmerican University of Puerto Rico · PRThe University of Sydney · AUPublic Health England · GBSeaWorld Entertainment · US

Funding

Developing techniques to generate the first transgenic opossumsR21OD022988 · OD · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI SEARS, KAREN E · 2016 to 2017
$426k
NIH HHS R21 OD022988
6 · The paper itself

Abstract

The opossum (Monodelphis domestica), with its sequenced genome, ease of laboratory care and experimental manipulation, and unique biology, is the most used laboratory marsupial. Using the mammalian methylation array, we generated DNA methylation data from n = 100 opossum samples from the ear, liver, and tail. We contrasted postnatal development and later aging effects in the opossum methylome with those in mouse (Mus musculus, C57BL/6 J strain) and other marsupial species such as Tasmanian devil, kangaroos, and wallabies. While the opossum methylome is similar to that of mouse during postnatal development, it is distinct from that shared by other mammals when it comes to the age-related gain of methylation at target sites of polycomb repressive complex 2. Our immunohistochemical staining results provide additional support for the hypothesis that PRC2 activity increases with later aging in mouse tissues but remains constant in opossum tissues. We present several epigenetic clocks for opossums that are distinguished by their compatibility with tissue type (pan-tissue and blood clock) and species (opossum and human). Two dual-species human-opossum pan-tissue clocks accurately measure chronological age and relative age, respectively. The human-opossum epigenetic clocks are expected to provide a significant boost to the attractiveness of opossum as a biological model. Additional epigenetic clocks for Tasmanian devil, red kangaroos and other species of the genus Macropus may aid species conservation efforts.

Indexed as

DNA MethylationMacropodidaeAnimalsEpigenesis, GeneticEpigenomicsMiceMice, Inbred C57BLAgingDevelopmentDNA methylationEpigenetic clockOpossum

Identifiers

PMID35449380
PMCPMC9213610
OpenAlexW4224290083

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.