Evidence map›Paper›PMID 35449367›Full record

ArticleMedical molecular morphology2022

A morphological study of adipose-derived stem cell sheets created with temperature-responsive culture dishes using scanning electron microscopy.

Yasuhiko Taki, Atsushi Fuku, Yuka Nakamura, Terutsugu Koya, Hironori Kitajima, Ikuhiro Tanida, Takashi Takaki, Kaori Nozaki, Hiroshi Sunami, Hiroaki Hirata and 11 more

Abstract read
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In one paragraph

Article in Medical molecular morphology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Yasuhiko TakiDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Atsushi FukuDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Yuka NakamuraMedical Research Institute, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Terutsugu KoyaCenter for Regenerative Medicine, Kanazawa Medical University Hospital, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Hironori KitajimaDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Ikuhiro TanidaGenome Biotechnology Laboratory, Kanazawa Institute of Technology, Yatsukaho, Hakusan, Ishikawa, 924-0838, Japan.
Takashi TakakiDivision of Electron Microscopy, Showa University, Shinagawa, Tokyo, 142-8555, Japan.
Kaori NozakiDepartment of Pharmacy, Kanazawa Medical University Hospital, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Hiroshi SunamiFaculty of Medicine, Advanced Medical Research Center, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, Okinawa, 903-0215, Japan.
Hiroaki HirataDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Yoshiyuki TachiDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Takeo ShimasakiMedical Research Institute, Department of Advanced Medicine, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Togen MasaujiDepartment of Pharmacy, Kanazawa Medical University Hospital, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Naoki YamamotoCenter for Clinical Trial and Research Support, Research Promotion and Support Headquarters, Fujita Health University, 1-98 Dengakugakubo, Kutsukake-cho, Toyoake, Aichi, 470-1192, Japan.
Yasuhito IshigakiMedical Research Institute, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Shigetaka ShimodairaCenter for Regenerative Medicine, Kanazawa Medical University Hospital, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Yusuke ShimizuDepartment of Plastic and Reconstructive Surgery, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Nakagami, Okinawa, 903-0215, Japan.
Toru IchisekiDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan. tsy-ichi@kanazawa-med.ac.jp.
Ayumi KaneujiDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.
Satoshi OsawaGenome Biotechnology Laboratory, Kanazawa Institute of Technology, Yatsukaho, Hakusan, Ishikawa, 924-0838, Japan.
Norio KawaharaDepartment of Orthopedic Surgery, Kanazawa Medical University, Uchinada, Kahoku, Ishikawa, 920-0293, Japan.

Funding

KAKENHI 21K09312
6 · The paper itself

Abstract

Adipose-derived stem cell (ADSC) sheets have potential to be effective in various therapies. In this study, we first demonstrated that a cell sheet composed of human ADSCs could be created using a new temperature-responsive culture dish from the DIC Corporation. The dish can cause detachment of adherent cells due to temperature changes, but a few morphological analyses have evaluated the presence or absence of damage on the detached surface of cell sheet. To characterize our ADSC sheet, we tried to observe the surface of ADSC sheets with scanning electron microscope (SEM) using the ionic liquid, which enables the rapid preparation of samples. No damage was found on the surface of the ADSC sheets on the side that had been in contact with the surface of the culture dishes. In addition, when the transcriptomes of the harvested cell sheets were compared with those of monolayer cultures, no up-regulation of cell death related genes were detected. These results propose that the detachment from temperature-responsive culture dish causes no serious damage on the prepared ADSC sheet. It is also suggested that the SEM with ionic liquids is a useful and rapid method for the analysis of ADSC sheets for therapy.

Indexed as

Adipose TissueStem CellsAdipocytesHumansMicroscopy, Electron, ScanningTemperatureAdipose-derived stem cellsCell sheetsIonic liquidsScanning electron microscopyTemperature-responsive culture dishes

Identifiers

PMID35449367

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.