ArticleScientific reports2022
Behavioral and slice electrophysiological assessment of DREADD ligand, deschloroclozapine (DCZ) in rats.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Off-target effects of DREADD ligands revealed by an anesthesia emergence paradigm in mice.Cell reports methods · 2026Article
- Chemogenetic inhibition of the anterior cingulate cortex increases safe decision-making in aged mice performing the Iowa Gambling Task.Psychopharmacology · 2026Article
- Lateral hypothalamic melanin concentrating hormone-expressing neurons both promote and are required for cue-potentiated feeding.Journal of neuroendocrinology · 2026Article
- Dichotomous Regulation of Social Behaviors by Nucleus Accumbens Neurons in Female and Male Syrian Hamsters.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026Article
- High-throughput assessment of vocal modulation following chemogenetic inhibition in songbirds.iScience · 2026Article
- Differential effects of acute versus repeated chemogenetic activation of the mesocorticolimbic dopamine pathway on cocaine choice in male and female TH:Cre rats.ASPET discovery · 2026Article
- Sparse memory ensembles set brain-wide network states to sustain learned associations.iScience · 2025Article
- GABAergic interneurons contribute to the fatal seizure phenotype of CLN2 disease mice.JCI insight · 2025Article
- Insular input to the prelimbic cortex underlies social affective behavior in rats.bioRxiv : the preprint server for biology · 2025Article
- Central amygdala astrocyte plasticity underlies GABAergic dysregulation in ethanol dependence.Translational psychiatry · 2025Article
- Contribution of Rat Insular Cortex to Stimulus-Guided Action.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025Article
- Article
- Dynamic responses of striatal cholinergic interneurons control behavioral flexibility.Science advances · 2024Article
- Punishment-resistant alcohol intake is mediated by the nucleus accumbens shell in female rats.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2024Article
- A disinhibitory microcircuit of the orbitofrontal cortex mediates cocaine preference in mice.Molecular psychiatry · 2024Article
- Forelimb motor recovery by modulating extrinsic and intrinsic signaling as well as neuronal activity after the cervical spinal cord injury.bioRxiv : the preprint server for biology · 2024Article
- Chemogenetic inhibition of the lateral hypothalamus effectively reduces food intake in rats in a translational proof-of-concept study.Scientific reports · 2024Article
- Identification of New Modulators and Inhibitors of Palmitoyl-Protein Thioesterase 1 for CLN1 Batten Disease and Cancer.ACS omega · 2024Article
- Imaging-based chemogenetics for dissecting neural circuits in nonhuman primates.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2024Review
- Exploring memory-related network via dorsal hippocampus suppression.Network neuroscience (Cambridge, Mass.) · 2024Article
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4 authors.
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Abstract
Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) have become a premier neuroscience research tool for enabling reversible manipulations of cellular activity following experimenter-controlled delivery of a DREADD-specific ligand. However, several DREADD ligands, e.g., clozapine-N-oxide (CNO), have metabolic and off-target effects that may confound experimental findings. New DREADD ligands aim to reduce metabolic and potential off-target effects while maintaining strong efficacy for the designer receptors. Recently a novel DREADD ligand, deschloroclozapine (DCZ), was shown to induce chemogenetic-mediated cellular and behavioral effects in mice and monkeys without detectable side effects. The goal of the present study was to examine the effectiveness of systemic DCZ for DREADD-based chemogenetic manipulations in behavioral and slice electrophysiological applications in rats. We demonstrate that a relatively low dose of DCZ (0.1 mg/kg) supports excitatory DREADD-mediated cFos induction, DREADD-mediated inhibition of a central amygdala-dependent behavior, and DREADD-mediated inhibition of neuronal activity in a slice electrophysiology preparation. In addition, we show that this dose of DCZ does not alter gross locomotor activity or induce a place preference/aversion in control rats without DREADD expression. Together, our findings support the use of systemic DCZ for DREADD-based manipulaations in rats, and provide evidence that DCZ is a superior alternative to CNO.
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