ArticleParasites & vectors2022
Prohemocytes are the main cells infected by dengue virus in Aedes aegypti and Aedes albopictus.
Article in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Entomological Surveillance and Vector Competence: A Crucial Front in Dengue Prevention.Current topics in microbiology and immunology · 2026Review
- Deep data-independent acquisition-based plasma proteomic profiling unveils distinct molecular features in dengue fever with neutropenia.Virologica Sinica · 2025Article
- Mosquito immune cells enhance dengue and Zika virus infection in Aedes aegypti.Nature communications · 2025Article
- Influence of blood feeding and infection on arthropod hemocytes.Current opinion in insect science · 2025Review
- Larvicidal potential of Knoevenagel adducts againstFuture medicinal chemistry · 2025Article
- Comparative evaluation of three herbal extracts on growth performance, immune response, and resistance against Vibrio parahaemolyticus in Litopenaeus vannamei.BMC veterinary research · 2025Article
- The making of a dangerous vector: factors shaping the vector competence ofFrontiers in cellular and infection microbiology · 2025Review
- A tangled threesome: understanding arbovirus infection inFrontiers in microbiology · 2023Review
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe primary disease vectors for dengue virus (DENV) transmission between humans are the mosquitoes Aedes aegypti and Aedes albopictus, with Ae. aegypti population size strongly correlated with DENV outbreaks. When a mosquito is infected with DENV, the virus migrates from the midgut to the salivary glands to complete the transmission cycle. How the virus crosses the hemocoel, resulting in systemic infection, is still unclear however. During viral infection and migration, the innate immune system is activated in defense. As part of cellular-mediated immunity, hemocytes are known to defend against bacteria and Plasmodium infection and may also participate in defending against DENV infection. Hemocytes are categorized into three cell types: prohemocytes, granulocytes, and oenocytoids. Here, we investigated which hemocytes can be infected by DENV and compare hemocyte infection between Ae. aegypti and Ae. albopictus.
methodsHemocytes were collected from Ae. aegypti and Ae. albopictus mosquitoes that were intrathoracically infected with DENV2-GFP. The collected hemocytes were then identified via Giemsa staining and examined microscopically for morphological differences and viral infection.
resultsAll three types of hemocytes were infected by DENV, though the predominantly infected cell type was prohemocytes. In Ae. aegypti, the highest and lowest infection rates at 7 days post infection occurred in prohemocytes and granulocytes, respectively. Prohemocytes were also the primary infection target of DENV in Ae. albopictus, with similar infection rates across the other two hemocyte groups. The ratios of hemocyte composition did not differ significantly between non-infected and infected mosquitoes for either species.
conclusionsIn this study, we showed that prohemocytes were the major type of hemocyte infected by DENV in both Ae. aegypti and Ae. albopictus. The infection rate of prohemocytes in Ae. albopictus was lower than that in Ae. aegypti, which may explain why systemic DENV infection in Ae. albopictus is less efficient than in Ae. aegypti and why Ae. albopictus is less correlated to dengue fever outbreaks. Future work in understanding the mechanisms behind these phenomena may help reduce arbovirus infection prevalence.
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