Evidence map›Paper›PMID 35448968›Full record

ArticleBMC genomic data2022

Whole genome sequencing and taxonomic profiling of two Pantoea sp. isolated from environmental samples in Israel.

Yehoudit Guedj-Dana, Inbar Cohen-Gihon, Ofir Israeli, Ohad Shifman, Tamar Aminov, Shahar Rotem, Raphael Ber, Anat Zvi

Abstract read
In one paragraph

Article in BMC genomic data, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Mechanism ofFrontiers in microbiology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yehoudit Guedj-DanaDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
Inbar Cohen-GihonDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
Ofir IsraeliDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
Ohad ShifmanDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
Tamar AminovDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
Shahar RotemDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
Raphael BerDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel.
Anat ZviDepartment of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness Ziona, Israel. anatz@iibr.gov.il.ORCID 0000-0003-3743-9642

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveAs part of a research aiming at the isolation of bacteria secreting growth inhibiting compounds, cultures of Francisella tularensis were implanted in environmental samples and monitored for inhibition zones on agar. Two antibiotic-like secreting bacteria were isolated, their genomic sequence was deciphered and taxonomic profiling analysis classified them as belonging to the Pantoea genus. DATA DESCRIPTION: Two bacterial isolates exhibiting growth inhibition zones to F. tularensis (LVS) were analyzed using the Oxford Nanopore Technology (ONT). Preliminary de novo assembly of the reads was performed, followed by taxonomic profiling based on Multi Locus Sequence Analysis (MLSA) and implementation of the Average Nucleotide Identity (ANI) measure. The genomic sequences resulted in the identification of two different Pantoea species, denoted EnvD and EnvH. Subsequent de novo genome assembly generated 5 and 10 contigs for EnvD and EnvH, respectively. The largest contig (4,008,183 bps and 3,740,753 bps for EnvD and EnvH, respectively), overlaps to a major extent to the chromosome of closely related Pantoea species. ANI values calculated for both isolates revealed two apparently new species of the Pantoea genus. Our study deciphered the identity of two bacteria producing antibiotic-like compounds, and the genomic sequence revealed they represent distinct Pantoea species.

Indexed as

PantoeaAnti-Bacterial AgentsIsraelPhylogenySequence Analysis, DNAWhole Genome SequencingAnti-Bacterial AgentsDe novo assemblyEnvironmental samplesOxford nanoporePantoeaTaxonomyWhole genome sequencing

Identifiers

PMID35448968
PMCPMC9022320

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.