ArticleThe American journal of pathology1987
HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.
Article in The American journal of pathology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
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Who cites it
97 citing papers in PubMed, 426 citations in OpenAlex.
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- Controlling the fibroblastic differentiation of mesenchymal stem cells via the combination of fibrous scaffolds and connective tissue growth factor.Tissue engineering. Part A · 2011Article
- Endosialin (Tem1) is a marker of tumor-associated myofibroblasts and tumor vessel-associated mural cells.The American journal of pathology · 2008Article
- Effects of heparin on the production of homocysteine-induced extracellular matrix metalloproteinase-2 in cultured rat vascular smooth muscle cells.The Canadian journal of cardiology · 2007Article
- Microvascular assembly and cell invasion in chick mesonephros grafted onto chorioallantoic membrane.Journal of anatomy · 2003Article
- The role of the epicardium and neural crest as extracardiac contributors to coronary vascular development.Texas Heart Institute journal · 2002Article
- Involvement of hydrogen peroxide in the differentiation of clonal HD-11EM cells into osteoclast-like cells.Journal of cellular physiology · 1998Article
- Expression of the peroxisome proliferator-activated receptor gamma (PPARgamma) in human atherosclerosis and regulation in macrophages by colony stimulating factors and oxidized low density lipoprotein.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.Anatomy and embryology · 1998Article
- Differential expression of laminin chains in the human major salivary gland.The Histochemical journal · 1998Article
- Onset of elastogenesis and downregulation of smooth muscle actin as distinguishing phenomena in artery differentiation in the chick embryo.Anatomy and embryology · 1996Article
- Nature and origin of the neointima in whole vessel wall organ culture of the human saphenous vein.Virchows Archiv : an international journal of pathology · 1996Article
- Experimental rabbit models of Chlamydia pneumoniae infection.The American journal of pathology · 1996Article
- Radiation induced intracranial leiomyosarcoma: its histopathological features.Acta neurochirurgica · 1996Article
- The role of angiogenic factor bFGF in the development of dural AVFs.Acta neurochirurgica · 1996Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A monoclonal antibody to muscle cell actin isotypes was produced and characterized. Immunocytochemical analysis of methanol-Carnoy's-fixed, paraffin-embedded human tissue revealed that this antibody, termed HHF35, reacts with skeletal muscle cells, cardiac muscle cells, smooth muscle cells, pericytes, and myoepithelial cells, but is nonreactive with endothelial, epithelial, neural, or connective tissue cells. When assayed by indirect immunofluorescence, HHF35 reacts with microfilament bundles from various cultured mammalian smooth muscle cells, but does not react with cultured human dermal fibroblasts or various epithelial tumor cell lines. In one-dimensional gel electrophoresis immunoblot experiments this antibody detects a 42-kd polypeptide from tissue extracts of uterus, ileum, aorta, diaphragm, and heart and extract from smooth muscle cells. The antibody also reacts with a comigrating 42-kd band of highly purified rabbit skeletal muscle actin. HHF35 is nonreactive on immunoblots of extracts from all tested nonmuscle cell extracts. Immunoelectrophoresis followed by immunoblotting performed in the presence of urea and reducing agents reveals recognition of the alpha isoelectrophoretic variant of actin from skeletal, cardiac, and smooth muscle sources and of the gamma variant from smooth muscle sources. Because HHF35 reacts with virtually all muscle cells, it will be useful as a marker for muscle and muscle-derived cells.
Indexed as
Identifiers
3544852PMC1899551W1564336366What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.