ArticleBiosensors2022
Revisiting the Resazurin-Based Sensing of Cellular Viability: Widening the Application Horizon.
Article in Biosensors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
61 citing papers in PubMed, 113 citations in OpenAlex.
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- Fluorescent probes as markers of cell envelope structure and function in halophilic archaea.Scientific reports · 2026Article
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- Enhanced Microbial Sensing via Resazurin Reduction Catalyzed by Graphene Oxide, A Versatile Approach for Diagnostics and Electrochemical Applications.Environmental toxicology · 2026Article
- Development of Resazurin-Based Assay for Rapid Evaluation of Sodium Hypochlorite Tolerance inFoods (Basel, Switzerland) · 2026Article
- Aggregation Tendency, Cellular Uptake, and Viability Effects of Structurally Distinct Carbazole-Phthalocyanine Gold Nanoconjugates.ACS organic & inorganic Au · 2026Article
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- Subcytotoxic Exposure to Avobenzone and Ethylhexyl Salicylate Induces microRNA Modulation and Stress-Responsive PI3K/AKT and MAPK Signaling in Differentiated SH-SY5Y Cells.International journal of molecular sciences · 2026Article
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- Milk-Derived Extracellular Vesicles Protect Bovine Oviduct Epithelial Cells from Oxidative Stress.Cells · 2025Article
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- A colorimetric method for detecting virulent bacteriophage to Vibrio cholerae in fecal and environmental samples.PLoS neglected tropical diseases · 2025Article
- Nanostructured Dual-Delivery System with Antioxidant and Synergistic Approach for Targeted Dermal Treatment.International journal of molecular sciences · 2025Article
- From Medical Imaging to Bioprinted Tissues: The Importance of Workflow Optimisation for Improved Cell Function.Expert reviews in molecular medicine · 2025Review
- Synthesis, Structure, DNA/BSA Binding, DNA Cleaving, Cytotoxic and SOD Mimetic Activities of Copper(II) Complexes Derived from Methoxybenzylamine Schiff Base Ligands.Molecules (Basel, Switzerland) · 2025Article
- Proteome changes associated with effect of high dose single-fractionation radiation on lung adenocarcinoma cell lines.Scientific reports · 2025Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Since 1991, the NAD(P)H-aided conversion of resazurin to fluorescent resorufin has been widely used to measure viability based on the metabolic activity in mammalian cell culture and primary cells. However, different research groups have used divergent assay protocols, scarcely reporting the systematic optimization of the assay. Here, we perform extensive studies to fine-tune the experimental protocols utilizing resazurin-based viability sensing. Specifically, we focus on (A) optimization of the assay dynamic range in individual cell lines for the correct measurement of cytostatic and cytotoxic properties of the compounds; (B) dependence of the dynamic range on the physical quantity detected (fluorescence intensity versus change of absorbance spectrum); (C) calibration of the assay for the correct interpretation of data measured in hypoxic conditions; and (D) possibilities for combining the resazurin assay with other methods including measurement of necrosis and apoptosis. We also demonstrate the enhanced precision and flexibility of the resazurin-based assay regarding the readout format and kinetic measurement mode as compared to the widely used analogous assay which utilizes tetrazolium dye MTT. The discussed assay optimization guidelines provide useful instructions for the beginners in the field and for the experienced scientists exploring new ways for measurement of cellular viability using resazurin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.