ArticleInsects2022
The Addition of Sterols and Cryoprotectants to Optimize a Diet Developed for
Article in Insects, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- Article
- Designing a Seasonal Acclimation Study Presents Challenges and Opportunities.Integrative organismal biology (Oxford, England) · 2022Article
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Authors and funding
3 authors.
Funding
Abstract
Various combinations and concentrations of cholesterol (C) and stigmasterol (S) were added into a base diet developed for Eldana saccharina. Survival of inoculated neonate was high on all diets (>92% at day 20 and >95% at day 27). Fastest larval development occurred on the minimum specification (MS) (+1.0 gS) and MS (+0.2 gC: 0.2 gS) diets (72 and 70% pupation respectively at day 20). Significantly slower development (15% pupation) occurred on the control diet at day 20. Female pupal weight increased when larvae fed on the MS (+0.1 gC), (+0.1 gS) and (+0.2 gC:0.2 gS) diets (0.2143 ± 0.00 g, 0.2271 ± 0.01 g and 0.2252 ± 0.01 g, respectively) as compared with the control diet (0.1886 ± 0.00 g). Adult emergence was significantly higher (100%) from the MS (+0.1 gS) and MS (+0.2 gC:0.2 gS) diets, as compared with the remaining sterol (95%) and control diets (97%). To potentially increase E. saccharina’s cold tolerance, inclusion of cryoprotectants L-proline (P) and trehalose (T) into the MS diet was investigated. Males from the MS (0.2 gP:0.2 gT), MS (0.5 gP:0.5 gT) and MS (1.0 gT) diets recovered fastest from chill coma treatment (204 ± 44 s, 215 ± 7 s and 215 ± 9 s, respectively) than those from the remaining cryoprotectant diets (305 ± 22 s). The addition of cryoprotectants severely reduced female fertility (<44%) when mated with non-chill coma exposed males. In contrast, eggs from females not exposed to chilling treatment were 84% fertile when mated with males from the same source. The MS (0.2 gC:0.2 gS) diet is the preferred choice to replace the currently used diet, reducing the larval growth period by 60% without negative effects on key life cycle parameters of E. saccharina.
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Registered trials
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