ArticleProceedings of the National Academy of Sciences of the United States of America2022
Circadian pacemaker neurons display cophasic rhythms in basal calcium level and in fast calcium fluctuations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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9 citing papers in PubMed, 16 citations in OpenAlex.
- Soma CaCurrent biology : CB · 2026Article
- Mutual coupling of neurons in the circadian master clock: What we can learn from fruit flies.Neurobiology of sleep and circadian rhythms · 2025Article
- Spatiotemporal computations in the insect celestial compass.Nature communications · 2025Article
- Circadian Rhythm Regulation by Pacemaker Neuron Chloride Oscillation in Flies.Physiology (Bethesda, Md.) · 2024Review
- Dynamic encoding of temperature in the central circadian circuit coordinates physiological activities.Nature communications · 2024Article
- Polyphasic circadian neural circuits drive differential activities in multiple downstream rhythmic centers.Current biology : CB · 2023Article
- An extra-clock ultradian brain oscillator sustains circadian timekeeping.Science advances · 2022Article
- Nested calcium dynamics support daily cell unity and diversity in the suprachiasmatic nuclei of free-behaving mice.PNAS nexus · 2022Article
- Comparative CaBiophysical reports · 2021Article
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3 authors at 1 institution in 1 country.
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Abstract
Circadian pacemaker neurons in the Drosophila brain display daily rhythms in the levels of intracellular calcium. These calcium rhythms are driven by molecular clocks and are required for normal circadian behavior. To study their biological basis, we employed genetic manipulations in conjunction with improved methods of in vivo light-sheet microscopy to measure calcium dynamics in individual pacemaker neurons over complete 24-h durations at sampling frequencies as high as 5 Hz. This technological advance unexpectedly revealed cophasic daily rhythms in basal calcium levels and in high-frequency calcium fluctuations. Further, we found that the rhythms of basal calcium levels and of fast calcium fluctuations reflect the activities of two proteins that mediate distinct forms of calcium fluxes. One is the inositol trisphosphate receptor (ITPR), a channel that mediates calcium fluxes from internal endoplasmic reticulum calcium stores, and the other is a T-type voltage-gated calcium channel, which mediates extracellular calcium influx. These results suggest that Drosophila molecular clocks regulate ITPR and T-type channels to generate two distinct but coupled rhythms in basal calcium and in fast calcium fluctuations. We propose that both internal and external calcium fluxes are essential for circadian pacemaker neurons to provide rhythmic outputs and thereby, regulate the activities of downstream brain centers.
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