ArticleBMC genomics2022
Genome sequencing of Inonotus obliquus reveals insights into candidate genes involved in secondary metabolite biosynthesis.
Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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33 citing papers in PubMed, 47 citations in OpenAlex.
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- From natural laboratory to drug discovery: Chemical structures, bioactivities, and biosynthesis of meroterpenoids fromChinese herbal medicines · 2026Review
- Independent evolution of betulin biosynthesis in Inonotus obliquus.Scientific reports · 2025Article
- NO Activates the Triterpenoid Biosynthetic Pathway inInternational journal of molecular sciences · 2025Article
- Whole-Genome Characterization ofJournal of fungi (Basel, Switzerland) · 2025Article
- Comparative Study of Chaga (International journal of molecular sciences · 2025Article
- Cloning and functional characterization of sesquiterpene synthase genes from Inonotus obliquus using a Saccharomyces cerevisiae expression system.World journal of microbiology & biotechnology · 2025Article
- Drought stress responses revealed by genomic and transcriptomic analyses of two macrofungi (IMA fungus · 2025Article
- Genome Sequencing Providing Molecular Evidence of Tetrapolar Mating System and Heterothallic Life Cycle for Edible and Medicinal MushroomJournal of fungi (Basel, Switzerland) · 2024Article
- Complete mitochondrial genome sequencing and phylogenetic analysis of Phellinus igniarius.Scientific reports · 2024Article
- Genome Sequencing and Metabolic Potential Analysis ofJournal of fungi (Basel, Switzerland) · 2024Article
- Genomic Features ofJournal of fungi (Basel, Switzerland) · 2024Article
- Genome analysis of the esca-associated Basidiomycetes Fomitiporia mediterranea, Fomitiporia polymorpha, Inonotus vitis, and Tropicoporus texanus reveals virulence factor repertoires characteristic of white-rot fungi.G3 (Bethesda, Md.) · 2024Article
- Secondary Metabolites ofJournal of fungi (Basel, Switzerland) · 2024Review
- Genome-wide characterization and metabolite profiling of Cyathus olla: insights into the biosynthesis of medicinal compounds.BMC genomics · 2024Article
- Research Progress of Bioactive Components inMolecules (Basel, Switzerland) · 2024Review
- Natural and engineered xylosyl products from microbial source.Natural products and bioprospecting · 2024Review
- The alkynyl-containing compounds from mushrooms and their biological activities.Natural products and bioprospecting · 2023Review
- Integrated omic profiling of the medicinal mushroom Inonotus obliquus under submerged conditions.BMC genomics · 2023Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundInonotus obliquus is an important edible and medicinal mushroom that was shown to have many pharmacological activities in preclinical trials, including anti-inflammatory, antitumor, immunomodulatory, and antioxidant effects. However, the biosynthesis of these pharmacological components has rarely been reported. The lack of genomic information has hindered further molecular characterization of this mushroom.
resultsIn this study, we report the genome of I. obliquus using a combined high-throughput Illumina NovaSeq with Oxford Nanopore PromethION sequencing platform. The de novo assembled 38.18 Mb I. obliquus genome was determined to harbor 12,525 predicted protein-coding genes, with 81.83% of them having detectable sequence similarities to others available in public databases. Phylogenetic analysis revealed the close evolutionary relationship of I. obliquus with Fomitiporia mediterranea and Sanghuangporus baumii in the Hymenochaetales clade. According to the distribution of reproduction-related genes, we predict that this mushroom possesses a tetrapolar heterothallic reproductive system. The I. obliquus genome was found to encode a repertoire of enzymes involved in carbohydrate metabolism, along with 135 cytochrome P450 proteins. The genome annotation revealed genes encoding key enzymes responsible for secondary metabolite biosynthesis, such as polysaccharides, polyketides, and terpenoids. Among them, we found four polyketide synthases and 20 sesquiterpenoid synthases belonging to four more types of cyclization mechanism, as well as 13 putative biosynthesis gene clusters involved in terpenoid synthesis in I. obliquus.
conclusionsTo the best of our knowledge, this is the first reported genome of I. obliquus; we discussed its genome characteristics and functional annotations in detail and predicted secondary metabolic biosynthesis-related genes, which provides genomic information for future studies on its associated molecular mechanism.
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