Evidence map›Paper›PMID 35443156›Full record

ArticleCell host & microbe2022

A secondary metabolite drives intraspecies antagonism in a gut symbiont that is inhibited by cell-wall acetylation.

Mustafa Özçam, Jee-Hwan Oh, Restituto Tocmo, Deepa Acharya, Shenwei Zhang, Theresa J Astmann, Mark Heggen, Silvette Ruiz-Ramírez, Fuyong Li, Christopher C Cheng and 6 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell host & microbe, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 25 citations in OpenAlex.

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  12. Draft genome sequence of probiotic bacteriumMicrobiology resource announcements · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 3 countries.

Mustafa ÖzçamDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Jee-Hwan OhDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Restituto TocmoDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Deepa AcharyaPharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, WI 53705, USA.
Shenwei ZhangDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Theresa J AstmannDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Mark HeggenDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Silvette Ruiz-RamírezDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA.
Fuyong LiDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Christopher C ChengDepartment of Biological Sciences, University of Alberta, Edmonton, AB T6G 2P5, Canada.
Eugenio VivasDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Federico E ReyDepartment of Bacteriology, University of Wisconsin-Madison, Madison, WI 53706, USA.
Jan ClaesenDepartment of Cardiovascular and Metabolic Sciences and Center for Microbiome and Human Health, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Tim S BugniPharmaceutical Sciences Division, University of Wisconsin-Madison, Madison, WI 53705, USA.
Jens WalterDepartment of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada; Department of Biological Sciences, University of Alberta, Edmonton, AB T6G 2P5, Canada; Department of Medicine and APC Microbiome Ireland, University College Cork, Cork T12 K8AF, Ireland; School of Microbiology, University College Cork, Cork T12 YT20, Ireland.
Jan-Peter van PijkerenDepartment of Food Science, University of Wisconsin-Madison, Madison, WI 53706, USA; Food Research Institute, University of Wisconsin-Madison, Madison, WI 53706, USA. Electronic address: vanpijkeren@wisc.edu.
University of Wisconsin–Madison · USUniversity of Alberta · CAAPC Microbiome Institute · IECleveland Clinic Lerner College of Medicine · US

Funding

Uncovering aryl polyene biology to identify new drug targets in Gram-negative bacterial pathogensR01AI153173 · NIAID · CLEVELAND CLINIC LERNER COM-CWRU · PI CLAESEN, JAN · 2021 to 2025
$2.0M
NIAID NIH HHS R01 AI153173
6 · The paper itself

Abstract

The mammalian microbiome encodes numerous secondary metabolite biosynthetic gene clusters; yet, their role in microbe-microbe interactions is unclear. Here, we characterized two polyketide synthase gene clusters (fun and pks) in the gut symbiont Limosilactobacillus reuteri. The pks, but not the fun, cluster encodes antimicrobial activity. Forty-one of 51 L. reuteri strains tested are sensitive to Pks products; this finding was independent of strains' host origin. Sensitivity to Pks was also established in intraspecies competition experiments in gnotobiotic mice. Comparative genome analyses between Pks-resistant and -sensitive strains identified an acyltransferase gene (act) unique to Pks-resistant strains. Subsequent cell-wall analysis of wild-type and act mutant strains showed that Act acetylates cell-wall components, providing resistance to Pks-mediated killing. Additionally, pks mutants lost their competitive advantage, while act mutants lost their Pks resistance in in vivo competition assays. These findings provide insight into how closely related gut symbionts can compete and co-exist in the gastrointestinal tract.

Indexed as

Multigene FamilyPolyketide SynthasesAcetylationAnimalsGastrointestinal TractGerm-Free LifeMammalsMicePolyketide Synthasesacetylationacyltransferaseantimicrobialbiosynthetic gene clustersgut symbiontLimosilactobacillus reuterimicrobial competitionmicrobial ecologypolyenepolyketidesecondary metabolite

Identifiers

PMID35443156
PMCPMC12476563
OpenAlexW4224224424

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.