Evidence map›Paper›PMID 35443134›Full record

ArticleACS chemical biology2023

Cell Surface Engineering Enables Surfaceome Profiling.

Zak Vilen, Abigail E Reeves, Timothy R O'Leary, Eugene Joeh, Naomi Kamasawa, Mia L Huang

Open access · greenAbstract read
In one paragraph

Article in ACS chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 16 citations in OpenAlex.

  1. Sortase A-Mediated Farnesylation of Cdc42ACS synthetic biology · 2026
    Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Review
  7. Review
  8. Proximity Labeling of Cell Surface Proteins via Cell Surface Remodeling.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  9. Article
  10. Mapping the cancer surface proteome in search of target antigens for immunotherapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2024
    Review
  11. Article
  12. Review
  13. Review
  14. Surfaceome Profiling Identifies Basigin-Chaperoned Protein Clients.Chembiochem : a European journal of chemical biology · 2023
    Article
  15. Review
  16. Article
  17. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Zak VilenDepartment of Molecular Medicine, Scripps Research, 120 Scripps Way, Jupiter, Florida 33458, United States.
Abigail E ReevesDepartment of Molecular Medicine, Scripps Research, 120 Scripps Way, Jupiter, Florida 33458, United States.
Timothy R O'LearyDepartment of Molecular Medicine, Scripps Research, 120 Scripps Way, Jupiter, Florida 33458, United States.
Eugene JoehDepartment of Molecular Medicine, Scripps Research, 120 Scripps Way, Jupiter, Florida 33458, United States.
Naomi KamasawaThe Imaging Center and Electron Microscopy Core Facility, Max Planck Florida Institute for Neuroscience, 1 Max Planck Way, Jupiter, Florida 33458, United States.
Mia L HuangDepartment of Molecular Medicine, Scripps Research, 120 Scripps Way, Jupiter, Florida 33458, United States.ORCID 0000-0001-9909-9554
Scripps Research Institute · USMax Planck Florida Institute for Neuroscience · US

Funding

Causes and functional consequences of chromatin evolutionR35GM124684 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI Mia Tauna Levine · 2017 to 2026
$3.5M
Bridging the Glycome and Proteome with Chemical BiologyR35GM142462 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI Mia L Huang · 2021 to 2026
$2.9M
Illuminating Galectin-3 Interactions to Disrupt Hepatic FibrosisR56DK126895 · NIDDK · SCRIPPS RESEARCH INSTITUTE, THE · PI HUANG, MIA L · 2021 to 2021
$490k
NIDDK NIH HHS R56 DK126895NIGMS NIH HHS R35 GM124684NIGMS NIH HHS R35 GM142462
6 · The paper itself

Abstract

Cell surface proteins (CSPs) are vital molecular mediators for cells and their extracellular environment. Thus, understanding which CSPs are displayed on cells, especially in different cell states, remains an important endeavor in cell biology. Here, we describe the integration of cell surface engineering with radical-mediated protein biotinylation to profile CSPs. This method relies on the prefunctionalization of cells with cholesterol lipid groups, followed by sortase-catalyzed conjugation with an APEX2 ascorbate peroxidase enzyme. In the presence of biotin-phenol and H

Indexed as

AdenocarcinomaCell MembraneMembrane ProteinsProteomicsAnimalsBiotinylationHumansHydrogen PeroxideMammalsPancreatic NeoplasmsStreptavidinHydrogen PeroxideMembrane ProteinsStreptavidin

Identifiers

PMID35443134
PMCPMC9901301
OpenAlexW4224228407

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.