ArticleThe Plant cell2022
Calcium-dependent protein kinase 16 phosphorylates and activates the aquaporin PIP2;2 to regulate reversible flower opening in Gentiana scabra.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed.
- The adaptive role and molecular mechanism of thermoregulated corolla opening and closure in the alpine species Gentiana rigescens.BMC plant biology · 2026Article
- Transcriptional and post-translational regulation of aquaporins in plants.Stress biology · 2026Review
- LcMYB306 regulates litchi fruit water loss and browning by inhibiting the expression ofHorticulture research · 2026Article
- Plant Robotics for Sustainable and Environmentally Friendly Robots: Insights from Actuation Characteristics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Calcium-Dependent Protein Kinases in Plants: Structure, Signaling, and Multifaceted Regulatory Roles in Development and Stress Adaptation.International journal of molecular sciences · 2026Review
- Genome-wide analysis of expansin and aquaporin genes and their association with auxin-related metabolites during flower opening inFrontiers in plant science · 2026Article
- The Gentiana rigescens genome and transcriptome for the investigation of flower repetitive blooming.BMC biology · 2025Article
- Genome-Wide Identification and Expression Analysis of Aquaporin Gene Family in Tea Plant (Plants (Basel, Switzerland) · 2025Article
- Osmotic stress triggers calcium-mediated expression of the aquaporin (TIP2;1) gene in ashwagandha (Withania somnifera L. Dunal).Molecular biology reports · 2025Article
- Scaffold protein RhCASPL1D1 stabilizes RhPIP2 aquaporins and promotes flower recovery after dehydration in rose (Horticulture research · 2025Article
- Branched-chain amino acid catabolism initiates volatile synthesis in Gentiana triflora.Planta · 2025Article
- Applications of the wheat germ cell-free protein synthesis system in plant biochemical studies.Plant biotechnology (Tokyo, Japan) · 2024Article
- LcMPK3 and LcMPK6 positively regulate fruitlet abscission in litchi.Molecular horticulture · 2024Article
- Genome-Wide Identification and Expression Pattern Profiling of the Aquaporin Gene Family in Papaya (International journal of molecular sciences · 2023Article
- The CALCINEURIN B-LIKE 4/CBL-INTERACTING PROTEIN 3 module degrades repressor JAZ5 during rose petal senescence.Plant physiology · 2023Article
- Genome-Wide Analysis of Aquaporins in Japanese Morning Glory (Plants (Basel, Switzerland) · 2023Article
- Exploring aquaporin functions during changes in leaf water potential.Frontiers in plant science · 2023Review
- De Novo Transcriptome Analysis Reveals Flowering-Related Genes That Potentially Contribute to Flowering-Time Control in the Japanese Cultivated GentianInternational journal of molecular sciences · 2022Article
- Masters of flower-bending: Aquaporins regulate flower re-opening.The Plant cell · 2022Article
- Isolation and Functional Analysis ofInternational journal of molecular sciences · 2022Article
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Abstract
Flower opening is important for successful pollination in many plant species, and some species repeatedly open and close their flowers. This is thought to be due to turgor pressure changes caused by water influx/efflux, which depends on osmotic oscillations in the cells. In some ornamental plants, water-transporting aquaporins, also known as plasma membrane intrinsic proteins (PIPs), may play an important role in flower opening. However, the molecular mechanism(s) involved in corolla movement are largely unknown. Gentian (Gentiana spp.) flowers undergo reversible movement in response to temperature and light stimuli; using gentian as a model, we showed that the Gentiana scabra aquaporins GsPIP2;2 and GsPIP2;7 regulate repeated flower opening. In particular, phosphorylation of a C-terminal serine residue of GsPIP2;2 is important for its transport activity and relates closely to the flower re-opening rate. Furthermore, GsPIP2;2 is phosphorylated and activated by the calcium (Ca2+)-dependent protein kinase GsCPK16, which is activated by elevated cytosolic Ca2+ levels in response to temperature and light stimuli. We propose that GsCPK16-dependent phosphorylation and activation of GsPIP2;2 regulate gentian flower re-opening, with stimulus-induced Ca2+ signals acting as triggers.
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