ArticleScientific reports2022
Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The trial behind it
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Who cites it
9 citing papers in PubMed, 19 citations in OpenAlex.
- DNase Promotes the Release of Sperm Trapped by NETs and Induces Important Changes in Donkey Sperm Motility Patterns.Animals : an open access journal from MDPI · 2026Article
- From reservoir to rendezvous: the journey of sperm through the oviduct.Reproduction, fertility, and development · 2026Review
- Sulfated Lewis A from the oviduct reservoir selectively binds to camel (Veterinary world · 2025Article
- Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct.Scientific reports · 2025Article
- Bottom-up approach to deciphering the targets of the ubiquitin-proteasome system in porcine sperm capacitation.Scientific reports · 2024Article
- Sperm in the Mammalian Female Reproductive Tract: Surfing Through the Tract to Try to Beat the Odds.Annual review of animal biosciences · 2024Review
- Sperm hyperactivation in the uterus and oviduct: a double-edged sword for sperm and maternal innate immunity toward fertility.Animal reproduction · 2024Review
- The Ubiquitin-Proteasome System Participates in Sperm Surface Subproteome Remodeling during Boar Sperm Capacitation.Biomolecules · 2023Article
- Molecular mechanisms of mammalian sperm capacitation, and its regulation by sodium-dependent secondary active transporters.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
Fertilizing sperm are retained by adhesion to specific glycans on the epithelium of the oviduct forming a reservoir before sperm are released from the reservoir so fertilization can ensue. Capacitated sperm lose affinity for the oviduct epithelium but the components of capacitation that are important for sperm release are uncertain. One important correlate of capacitation is the development of hyperactivated motility. Hyperactivation is characterized by asymmetrical flagellar beating with high beat amplitude. We tested whether the development of full-type asymmetrical motility was sufficient to release sperm from immobilized oviduct glycans. Sperm hyperactivation was induced by four different compounds, a cell-permeable cAMP analog (cBiMPS), CatSper activators (4-aminopyridine and procaine), and an endogenous steroid (progesterone). Using standard analysis (CASA) and direct visualization with high-speed video microscopy, we first confirmed that all four compounds induced hyperactivation. Subsequently, sperm were allowed to bind to immobilized oviduct glycans, and compounds or vehicle controls were added. All compounds caused sperm release from immobilized glycans, demonstrating that hyperactivation was sufficient to release sperm from oviduct cells and immobilized glycans. Pharmacological inhibition of the non-genomic progesterone receptor and CatSper diminished sperm release from oviduct glycans. Inhibition of the proteolytic activities of the ubiquitin-proteasome system (UPS), implicated in the regulation of sperm capacitation, diminished sperm release in response to all hyperactivation inducers. In summary, induction of sperm hyperactivation was sufficient to induce sperm release from immobilized oviduct glycans and release was dependent on CatSper and the UPS.
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Registered trials
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